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2023

O'Boyle, Bridget. (2023) Scientists with Impact: Interview with Ben Engel.

Bregy, Irina and Radecke, Julika and Noga, Akira and van den Hoek, Hugo and Kern, Mara and Haenni, Beat and Engel, Benjamin D. and Siebert, C. Alistair and Ishikawa, Takashi and Zuber, Benoît and Ochsenreiter, Torsten.. (2023) Cryo-electron tomography sheds light on the elastic nature 2 of the Trypanosoma brucei tripartite attachment complex.

Kulaj, Konxhe and Harger, Alexandra and Bauer, Michaela and Caliskan, Özüm S. and Gupta, Tilak Kumar and Chiang, Dapi Menglin and Milbank, Edward and Reber, Josefine and Karlas, Angelos and Kotzbeck, Petra and Sailer, David N. and Volta, Francesco and Lutter, Dominik and Prakash, Sneha and Merl-Pham, Juliane and Ntziachristos, Vasilis and Hauner, Hans and Pfaffl, Michael W. and Tschöp, Matthias H. and Müller, Timo D. and Hauck, Stefanie M. and Engel, Benjamin D. and Gerdes, Jantje M. and Pfluger, Paul T. and Krahmer, Natalie and Stemmer, Kerstin. (2023) Adipocyte-derived extracellular vesicles increase insulin secretion through transport of insulinotropic protein cargo. Nature Communications, 14 (1). p. 709.

Khavnekar, Sagar and Kelley, Ron and Waltz, Florent and Wietrzynski, Wojciech and Zhang, Xianjun and Obr, Martin and Tagiltsev, Grigory and Beck, Florian and Wan, William and Briggs, John and Engel, Ben and Plitzko, Juergen and Kotecha, Abhay. (2023) Towards the Visual Proteomics of C. reinhardtii using High-throughput Collaborative in situ Cryo-ET. Microscopy and Microanalysis, 29 (Supplement 1). pp. 961-963.

Righetto, Ricardo D. and Engel, Benjamin D.. (2023) Visualizing a Carbon-Fixing Nanowire Inside Bacteria. Chimia, 77 (5). p. 348.

Goodenough, Ursula and Engel, Benjamin D.. (2023) Cell Ultrastructure. In: The Chlamydomonas Sourcebook, 1: Introduction to Chlamydomonas and Its Laboratory Use. St. Louis, MO, USA, pp. 17-40.

Wietrzynski, Wojciech and Engel, Benjamin D.. (2023) Supramolecular Organization of Chloroplast Membranes. In: The Chlamydomonas Sourcebook, 2: Organellar and Metabolic Processes. St. Louis, MO, USA, pp. 763-785.

2022

van den Hoek, Hugo and Klena, Nikolai and Jordan, Mareike A. and Alvarez Viar, Gonzalo and Righetto, Ricardo D. and Schaffer, Miroslava and Erdmann, Philipp S. and Wan, William and Geimer, Stefan and Plitzko, Jürgen M. and Baumeister, Wolfgang and Pigino, Gaia and Hamel, Virginie and Guichard, Paul and Engel, Benjamin D.. (2022) In situ architecture of the ciliary base reveals the stepwise assembly of intraflagellar transport train. Science, 377 (6605). pp. 543-548.

Dietrich, Helge M. and Righetto, Ricardo D. and Kumar, Anuj and Wietrzynski, Wojciech and Trischler, Raphael and Schuller, Sandra K. and Wagner, Jonathan and Schwarz, Fabian M. and Engel, Benjamin D. and Müller, Volker and Schuller, Jan M.. (2022) Membrane-anchored HDCR nanowires drive hydrogen-powered CO2 fixation. Nature, 607 (7920). pp. 823-830.

Lamm, Lorenz and Righetto, Ricardo D. and Wietrzynski, Wojciech and Pöge, Matthias and Martinez-Sanchez, Antonio and Peng, Tingying and Engel, Benjamin D.. (2022) MemBrain: a deep learning-aided pipeline for detection of membrane proteins in cryo-electron tomograms. Computer Methods and Programs in Biomedicine, 224. p. 106990.

Righetto, Ricardo D. and Engel, Benjamin D.. (2022) Expanding the arsenal of bacterial spearguns. Nature Microbiology, 7 (3). pp. 363-364.

2021

Waltz, Florent and Salinas-Giegé, Thalia and Englmeier, Robert and Meichel, Herrade and Soufari, Heddy and Kuhn, Lauriane and Pfeffer, Stefan and Förster, Friedrich and Engel, Benjamin D. and Giegé, Philippe and Drouard, Laurence and Hashem, Yaser. (2021) How to build a ribosome from RNA fragments in Chlamydomonas mitochondria. Nature Communications, 12 (1). p. 7176.

Moebel, Emmanuel and Martinez-Sanchez, Antonio and Lamm, Lorenz and Righetto, Ricardo D. and Wietrzynski, Wojciech and Albert, Sahradha and Larivière, Damien and Fourmentin, Eric and Pfeffer, Stefan and Ortiz, Julio and Baumeister, Wolfgang and Peng, Tingying and Engel, Benjamin D. and Kervrann, Charles. (2021) Deep learning improves macromolecule identification in 3D cellular cryo-electron tomograms. Nature Methods, 18 (11). pp. 1386-1394.

Gupta, Tilak Kumar and Klumpe, Sven and Gries, Karin and Heinz, Steffen and Wietrzynski, Wojciech and Ohnishi, Norikazu and Niemeyer, Justus and Spaniol, Benjamin and Schaffer, Miroslava and Rast, Anna and Ostermeier, Matthias and Strauss, Mike and Plitzko, Jürgen M. and Baumeister, Wolfgang and Rudack, Till and Sakamoto, Wataru and Nickelsen, Jörg and Schuller, Jan M. and Schroda, Michael and Engel, Benjamin D.. (2021) Structural basis for VIPP1 oligomerization and maintenance of thylakoid membrane integrity. Cell, 184 (14). pp. 3643-3659.e23.

Wietrzynski, Wojciech and Engel, Benjamin D.. (2021) Chlorophyll biogenesis sees the light. Nature Plants. Scientific Reports, 7 (4). pp. 380-381.

Zabret, Jure and Bohn, Stefan and Schuller, Sandra K. and Arnolds, Oliver and Möller, Madeline and Meier-Credo, Jakob and Liauw, Pasqual and Chan, Aaron and Tajkhorshid, Emad and Langer, Julian D. and Stoll, Raphael and Krieger-Liszkay, Anja and Engel, Benjamin D. and Rudack, Till and Schuller, Jan M. and Nowaczyk, Marc M.. (2021) Structural insights into photosystem II assembly. Nature Plants. Scientific Reports, 7 (4). pp. 524-538.

2020

He, Shan and Chou, Hui-Ting and Matthies, Doreen and Wunder, Tobias and Meyer, Moritz T. and Atkinson, Nicky and Martinez-Sanchez, Antonio and Jeffrey, Philip D. and Port, Sarah A. and Patena, Weronika and He, Guanhua and Chen, Vivian K. and Hughson, Frederick M. and McCormick, Alistair J. and Mueller-Cajar, Oliver and Engel, Benjamin D. and Yu, Zhiheng and Jonikas, Martin C.. (2020) The structural basis of Rubisco phase separation in the pyrenoid. Nature Plants. Scientific Reports, 6 (12). pp. 1480-1490.

Wietrzynski, Wojciech and Schaffer, Miroslava and Tegunov, Dimitry and Albert, Sahradha and Kanazawa, Atsuko and Plitzko, Jürgen M. and Baumeister, Wolfgang and Engel, Benjamin D.. (2020) Charting the native architecture of Chlamydomonas thylakoid membranes with single-molecule precision. eLife, 9. e53740.

Le Guennec, Maeva and Klena, Nikolai and Gambarotto, Davide and Laporte, Marine H. and Tassin, Anne-Marie and van den Hoek, Hugo and Erdmann, Philipp S. and Schaffer, Miroslava and Kovacik, Lubomir and Borgers, Susanne and Goldie, Kenneth N. and Stahlberg, Henning and Bornens, Michel and Azimzadeh, Juliette and Engel, Benjamin D. and Hamel, Virginie and Guichard, Paul. (2020) A helical inner scaffold provides a structural basis for centriole cohesion. Science Advances, 6 (7). eaaz4137.

Theis, Jasmine and Niemeyer, Justus and Schmollinger, Stefan and Ries, Fabian and Rütgers, Mark and Gupta, Tilak Kumar and Sommer, Frederik and Muranaka, Ligia Segatto and Venn, Benedikt and Schulz-Raffelt, Miriam and Willmund, Felix and Engel, Benjamin D. and Schroda, Michael. (2020) VIPP2 interacts with VIPP1 and HSP22E/F at chloroplast membranes and modulates a retrograde signal for HSP22E/F gene expression. Plant, Cell and Environment, 43 (5). pp. 1212-1229.

Albert, Sahradha and Wietrzynski, Wojciech and Lee, Chia-Wei and Schaffer, Miroslava and Beck, Florian and Schuller, Jan M. and Salomé, Patrice A. and Plitzko, Jürgen M. and Baumeister, Wolfgang and Engel, Benjamin D.. (2020) Direct visualization of degradation microcompartments at the ER membrane. Proceedings of the National Academy of Sciences of the United States of America, 117 (2). pp. 1069-1080.

2019

Rast, Anna and Schaffer, Miroslava and Albert, Sahradha and Wan, William and Pfeffer, Stefan and Beck, Florian and Plitzko, Jürgen M. and Nickelsen, Jörg and Engel, Benjamin D.. (2019) Biogenic regions of cyanobacterial thylakoids form contact sites with the plasma membrane. Nature Plants. Scientific Reports, 5 (4). pp. 436-446.

Chicano, Andrea and Crosas, Eva and Otón, Joaquín and Melero, Roberto and Engel, Benjamin D. and Daban, Joan-Ramon. (2019) Frozen-hydrated chromatin from metaphase chromosomes has an interdigitated multilayer structure. The EMBO Journal, 38 (7). e99769.

Schuller, Jan M. and Birrell, James A. and Tanaka, Hideaki and Konuma, Tsuyoshi and Wulfhorst, Hannes and Cox, Nicholas and Schuller, Sandra K. and Thiemann, Jacqueline and Lubitz, Wolfgang and Sétif, Pierre and Ikegami, Takahisa and Engel, Benjamin D. and Kurisu, Genji and Nowaczyk, Marc M.. (2019) Structural adaptations of photosynthetic complex I enable ferredoxin-dependent electron transfer. Science, 363 (6424). pp. 257-260.

Craig, Evan W. and Mueller, David M. and Bigge, Brae M. and Schaffer, Miroslava and Engel, Benjamin D. and Avasthi, Prachee. (2019) The elusive actin cytoskeleton of a green alga expressing both conventional and divergent actins. Molecular Biology of the Cell, 30 (22). pp. 2827-2837.

Schaffer, Miroslava and Pfeffer, Stefan and Mahamid, Julia and Kleindiek, Stephan and Laugks, Tim and Albert, Sahradha and Engel, Benjamin D. and Rummel, Andreas and Smith, Andrew J. and Baumeister, Wolfgang and Plitzko, Juergen M.. (2019) A cryo-FIB lift-out technique enables molecular-resolution cryo-ET within native Caenorhabditis elegans tissue. Nature Methods, 16 (8). pp. 757-762.

Theis, Jasmine and Gupta, Tilak Kumar and Klingler, Johannes and Wan, William and Albert, Sahradha and Keller, Sandro and Engel, Benjamin D. and Schroda, Michael. (2019) VIPP1 rods engulf membranes containing phosphatidylinositol phosphates. Scientific Reports, 9 (1). p. 8725.

2018

Kovtun, Oleksiy and Leneva, Natalya and Bykov, Yury S. and Ariotti, Nicholas and Teasdale, Rohan D. and Schaffer, Miroslava and Engel, Benjamin D. and Owen, David J. and Briggs, John A. G. and Collins, Brett M.. (2018) Structure of the membrane-assembled retromer coat determined by cryo-electron tomography. Nature, 561 (7724). pp. 561-564.

Delarue, M. and Brittingham, G. P. and Pfeffer, S. and Surovtsev, I. V. and Pinglay, S. and Kennedy, K. J. and Schaffer, M. and Gutierrez, J. I. and Sang, D. and Poterewicz, G. and Chung, J. K. and Plitzko, J. M. and Groves, J. T. and Jacobs-Wagner, C. and Engel, B. D. and Holt, L. J.. (2018) mTORC1 controls phase separation and the biophysical properties of the cytoplasm by tuning crowding. Cell, 174 (2). pp. 338-349.e20.

Mosalaganti, Shyamal and Kosinski, Jan and Albert, Sahradha and Schaffer, Miroslava and Strenkert, Daniela and Salomé, Patrice A. and Merchant, Sabeeha S. and Plitzko, Jürgen M. and Baumeister, Wolfgang and Engel, Benjamin D. and Beck, Martin. (2018) In situ architecture of the algal nuclear pore complex. Nature Communications, 9 (1). p. 2361.

2017

Albert, Sahradha and Schaffer, Miroslava and Beck, Florian and Mosalaganti, Shyamal and Asano, Shoh and Thomas, Henry F. and Plitzko, Jürgen M. and Beck, Martin and Baumeister, Wolfgang and Engel, Benjamin D.. (2017) Proteasomes tether to two distinct sites at the nuclear pore complex. Proceedings of the National Academy of Sciences of the United States of America, 114 (52). pp. 13726-13731.

Bykov, Yury S. and Schaffer, Miroslava and Dodonova, Svetlana O. and Albert, Sahradha and Plitzko, Jürgen M. and Baumeister, Wolfgang and Engel, Benjamin D. and Briggs, John Ag. (2017) The structure of the COPI coat determined within the cell. eLife, 6. e32493.

Freeman Rosenzweig, Elizabeth S. and Xu, Bin and Kuhn Cuellar, Luis and Martinez-Sanchez, Antonio and Schaffer, Miroslava and Strauss, Mike and Cartwright, Heather N. and Ronceray, Pierre and Plitzko, Jürgen M. and Förster, Friedrich and Wingreen, Ned S. and Engel, Benjamin D. and Mackinder, Luke C. M. and Jonikas, Martin C.. (2017) The eukaryotic CO2-concentrating organelle is liquid-like and exhibits dynamic reorganization. Cell, 171 (1). pp. 148-162.e19.

Albanese, Pascal and Melero, Roberto and Engel, Benjamin D. and Grinzato, Alessandro and Berto, Paola and Manfredi, Marcello and Chiodoni, Angelica and Vargas, Javier and Sorzano, Carlos Óscar Sánchez and Marengo, Emilio and Saracco, Guido and Zanotti, Giuseppe and Carazo, Jose-Maria and Pagliano, Cristina. (2017) Pea PSII-LHCII supercomplexes form pairs by making connections across the stromal gap. Scientific Reports, 7 (1). p. 10067.

Pfeffer, Stefan and Dudek, Johanna and Schaffer, Miroslava and Ng, Bobby G. and Albert, Sahradha and Plitzko, Jürgen M. and Baumeister, Wolfgang and Zimmermann, Richard and Freeze, Hudson H. and Engel, Benjamin D. and Förster, Friedrich. (2017) Dissecting the molecular organization of the translocon-associated protein complex. Nature Communications, 8. p. 14516.

Schaffer, Miroslava and Mahamid, Julia and Engel, Benjamin D. and Laugks, Tim and Baumeister, Wolfgang and Plitzko, Jürgen M.. (2017) Optimized cryo-focused ion beam sample preparation aimed at in situ structural studies of membrane proteins. Journal of structural biology, 197 (2). pp. 73-82.

2016

Asano, Shoh and Engel, Benjamin D. and Baumeister, Wolfgang. (2016) In situ cryo-electron tomography: a post-reductionist approach to structural biology. Journal of Molecular Biology, 428 (2 Pt A). pp. 332-343.

2015

Schaffer, Miroslava and Engel, Benjamin D. and Laugks, Tim and Mahamid, Julia and Plitzko, Jürgen M. and Baumeister, Wolfgang. (2015) Cryo-focused ion beam sample preparation for imaging vitreous cells by cryo-electron tomography. Bio-protocol, 5 (17). e1575.

Engel, Benjamin D. and Schaffer, Miroslava and Albert, Sahradha and Asano, Shoh and Plitzko, Jürgen M. and Baumeister, Wolfgang. (2015) In situ structural analysis of Golgi intracisternal protein arrays. Proceedings of the National Academy of Sciences of the United States of America, 112 (36). pp. 11264-11269.

Engel, Benjamin D. and Schaffer, Miroslava and Kuhn Cuellar, Luis and Villa, Elizabeth and Plitzko, Jürgen M. and Baumeister, Wolfgang. (2015) Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography. eLife, 4. e04889.

2014

Bhogaraju, Sagar and Weber, Kristina and Engel, Benjamin D. and Lechtreck, Karl-Ferdinand and Lorentzen, Esben. (2014) Getting tubulin to the tip of the cilium: one IFT train, many different tubulin cargo-binding sites? BioEssays : news and reviews in molecular, cellular and developmental biology, 36 (5). pp. 463-467.

2013

Shih, Sheng Min and Engel, Benjamin D. and Kocabas, Fatih and Bilyard, Thomas and Gennerich, Arne and Marshall, Wallace F. and Yildiz, Ahmet. (2013) Intraflagellar transport drives flagellar surface motility. eLife, 2. e00744.

Bhogaraju, Sagar and Engel, Benjamin D. and Lorentzen, Esben. (2013) Intraflagellar transport complex structure and cargo interactions. Cilia, 2 (1). p. 10.

2012

Engel, Benjamin D. and Ishikawa, Hiroaki and Wemmer, Kimberly A. and Geimer, Stefan and Wakabayashi, Ken-ichi and Hirono, Masafumi and Craige, Branch and Pazour, Gregory J. and Witman, George B. and Kamiya, Ritsu and Marshall, Wallace F.. (2012) The role of retrograde intraflagellar transport in flagellar assembly, maintenance, and function. Journal of Cell Biology, 199 (1). pp. 151-167.

Rigort, Alexander and Villa, Elizabeth and Bäuerlein, Felix J. B. and Engel, Benjamin D. and Plitzko, Jürgen M.. (2012) Integrative approaches for cellular cryo-electron tomography: correlative imaging and focused ion beam micromachining. Methods in cell biology, 111. pp. 259-281.

Mizuno, Naoko and Taschner, Michael and Engel, Benjamin D. and Lorentzen, Esben. (2012) Structural studies of ciliary components. Journal of Molecular Biology, 422 (2). pp. 163-180.

2011

Engel, Benjamin D. and Ishikawa, Hiroaki and Feldman, Jessica L. and Wilson, Christopher W. and Chuang, Pao-Tien and Snedecor, June and Williams, Janice and Sun, Zhaoxia and Marshall, Wallace F.. (2011) A cell-based screen for inhibitors of flagella-driven motility in Chlamydomonas reveals a novel modulator of ciliary length and retrograde actin flow. Cell Motility and the Cytoskeleton , 68 (3). pp. 188-203.

2009

Engel, Benjamin D. and Ludington, William B. and Marshall, Wallace F.. (2009) Intraflagellar transport particle size scales inversely with flagellar length: revisiting the balance-point length control model. Journal of Cell Biology, 187 (1). pp. 81-89.

Engel, Benjamin D. and Lechtreck, Karl-Ferdinand and Sakai, Tsuyoshi and Ikebe, Mitsuo and Witman, George B. and Marshall, Wallace F.. (2009) Total internal reflection fluorescence (TIRF) microscopy of Chlamydomonas flagella. Methods in cell biology, 93. pp. 157-177.