Total internal reflection fluorescence (TIRF) microscopy of Chlamydomonas flagella

Engel, Benjamin D. and Lechtreck, Karl-Ferdinand and Sakai, Tsuyoshi and Ikebe, Mitsuo and Witman, George B. and Marshall, Wallace F.. (2009) Total internal reflection fluorescence (TIRF) microscopy of Chlamydomonas flagella. Methods in cell biology, 93. pp. 157-177.

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Official URL: https://edoc.unibas.ch/89510/

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The eukaryotic flagellum is host to a variety of dynamic behaviors, including flagellar beating, the motility of glycoproteins in the flagellar membrane, and intraflagellar transport (IFT), the bidirectional traffic of protein particles between the flagellar base and tip. IFT is of particular interest, as it plays integral roles in flagellar length control, cell signaling, development, and human disease. However, our ability to understand dynamic flagellar processes such as IFT is limited in large part by the fidelity with which we can image these behaviors in living cells. This chapter introduces the application of total internal reflection fluorescence (TIRF) microscopy to visualize the flagella of Chlamydomonas reinhardtii. The advantages and challenges of TIRF are discussed in comparison to confocal and differential interference contrast techniques. This chapter also reviews current IFT insights gleaned from TIRF microscopy of Chlamydomonas and provides an outlook on the future of the technique, with particular emphasis on combining TIRF with other emerging imaging technologies.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology and Biophysics (Engel)
UniBasel Contributors:Engel, Ben
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:17 Aug 2022 12:41
Deposited On:17 Aug 2022 12:41

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