The structural basis of Rubisco phase separation in the pyrenoid

He, Shan and Chou, Hui-Ting and Matthies, Doreen and Wunder, Tobias and Meyer, Moritz T. and Atkinson, Nicky and Martinez-Sanchez, Antonio and Jeffrey, Philip D. and Port, Sarah A. and Patena, Weronika and He, Guanhua and Chen, Vivian K. and Hughson, Frederick M. and McCormick, Alistair J. and Mueller-Cajar, Oliver and Engel, Benjamin D. and Yu, Zhiheng and Jonikas, Martin C.. (2020) The structural basis of Rubisco phase separation in the pyrenoid. Nature Plants. Scientific Reports, 6 (12). pp. 1480-1490.

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Official URL: https://edoc.unibas.ch/89578/

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Approximately one-third of global CO; 2; fixation occurs in a phase-separated algal organelle called the pyrenoid. The existing data suggest that the pyrenoid forms by the phase separation of the CO; 2; -fixing enzyme Rubisco with a linker protein; however, the molecular interactions underlying this phase separation remain unknown. Here we present the structural basis of the interactions between Rubisco and its intrinsically disordered linker protein Essential Pyrenoid Component 1 (EPYC1) in the model alga Chlamydomonas reinhardtii. We find that EPYC1 consists of five evenly spaced Rubisco-binding regions that share sequence similarity. Single-particle cryo-electron microscopy of these regions in complex with Rubisco indicates that each Rubisco holoenzyme has eight binding sites for EPYC1, one on each Rubisco small subunit. Interface mutations disrupt binding, phase separation and pyrenoid formation. Cryo-electron tomography supports a model in which EPYC1 and Rubisco form a codependent multivalent network of specific low-affinity bonds, giving the matrix liquid-like properties. Our results advance the structural and functional understanding of the phase separation underlying the pyrenoid, an organelle that plays a fundamental role in the global carbon cycle.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology and Biophysics (Engel)
UniBasel Contributors:Engel, Ben
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Research
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:06 Sep 2022 10:29
Deposited On:23 Aug 2022 09:02

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