In situ architecture of the ciliary base reveals the stepwise assembly of intraflagellar transport train

van den Hoek, Hugo and Klena, Nikolai and Jordan, Mareike A. and Alvarez Viar, Gonzalo and Righetto, Ricardo D. and Schaffer, Miroslava and Erdmann, Philipp S. and Wan, William and Geimer, Stefan and Plitzko, Jürgen M. and Baumeister, Wolfgang and Pigino, Gaia and Hamel, Virginie and Guichard, Paul and Engel, Benjamin D.. (2022) In situ architecture of the ciliary base reveals the stepwise assembly of intraflagellar transport train. Science, 377 (6605). pp. 543-548.

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Official URL: https://edoc.unibas.ch/89569/

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The cilium is an antenna-like organelle that performs numerous cellular functions, including motility, sensing, and signaling. The base of the cilium contains a selective barrier that regulates the entry of large intraflagellar transport (IFT) trains, which carry cargo proteins required for ciliary assembly and maintenance. However, the native architecture of the ciliary base and the process of IFT train assembly remain unresolved. In this work, we used in situ cryo-electron tomography to reveal native structures of the transition zone region and assembling IFT trains at the ciliary base in Chlamydomonas. We combined this direct cellular visualization with ultrastructure expansion microscopy to describe the front-to-back stepwise assembly of IFT trains: IFT-B forms the backbone, onto which bind IFT-A, dynein-1b, and finally kinesin-2 before entry into the cilium.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology and Biophysics (Engel)
UniBasel Contributors:van den Hoek, Hugo Guus and Diogo Righetto, Ricardo and Engel, Ben
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Association for the Advancement of Science
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:23 Aug 2022 10:16
Deposited On:23 Aug 2022 10:16

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