Charting the native architecture of Chlamydomonas thylakoid membranes with single-molecule precision

Wietrzynski, Wojciech and Schaffer, Miroslava and Tegunov, Dimitry and Albert, Sahradha and Kanazawa, Atsuko and Plitzko, Jürgen M. and Baumeister, Wolfgang and Engel, Benjamin D.. (2020) Charting the native architecture of Chlamydomonas thylakoid membranes with single-molecule precision. eLife, 9. e53740.

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Official URL: https://edoc.unibas.ch/89580/

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Thylakoid membranes scaffold an assortment of large protein complexes that work together to harness the energy of light. It has been a longstanding challenge to visualize how the intricate thylakoid network organizes these protein complexes to finely tune the photosynthetic reactions. Previously, we used in situ cryo-electron tomography to reveal the native architecture of thylakoid membranes (Engel et al., 2015). Here, we leverage technical advances to resolve the individual protein complexes within these membranes. Combined with a new method to visualize membrane surface topology, we map the molecular landscapes of thylakoid membranes inside green algae cells. Our tomograms provide insights into the molecular forces that drive thylakoid stacking and reveal that photosystems I and II are strictly segregated at the borders between appressed and non-appressed membrane domains. This new approach to charting thylakoid topology lays the foundation for dissecting photosynthetic regulation at the level of single protein complexes within the cell.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology and Biophysics (Engel)
UniBasel Contributors:Wietrzynski, Wojciech and Engel, Ben
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:eLife Sciences Publications
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:23 Aug 2022 10:44
Deposited On:23 Aug 2022 10:44

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