Biogenic regions of cyanobacterial thylakoids form contact sites with the plasma membrane

Rast, Anna and Schaffer, Miroslava and Albert, Sahradha and Wan, William and Pfeffer, Stefan and Beck, Florian and Plitzko, Jürgen M. and Nickelsen, Jörg and Engel, Benjamin D.. (2019) Biogenic regions of cyanobacterial thylakoids form contact sites with the plasma membrane. Nature Plants. Scientific Reports, 5 (4). pp. 436-446.

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Official URL: https://edoc.unibas.ch/89551/

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Little is known about how the photosynthetic machinery is arranged in time and space during the biogenesis of thylakoid membranes. Using in situ cryo-electron tomography to image the three-dimensional architecture of the cyanobacterium Synechocystis, we observed that the tips of multiple thylakoids merge to form a substructure called the 'convergence membrane'. This high-curvature membrane comes into close contact with the plasma membrane at discrete sites. We generated subtomogram averages of 70S ribosomes and array-forming phycobilisomes, then mapped these structures onto the native membrane architecture as markers for protein synthesis and photosynthesis, respectively. This molecular localization identified two distinct biogenic regions in the thylakoid network: thylakoids facing the cytosolic interior of the cell that were associated with both marker complexes, and convergence membranes that were decorated by ribosomes but not phycobilisomes. We propose that the convergence membranes perform a specialized biogenic function, coupling the synthesis of thylakoid proteins with the integration of cofactors from the plasma membrane and the periplasmic space.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology and Biophysics (Engel)
UniBasel Contributors:Engel, Ben
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Research
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:24 Jan 2023 13:25
Deposited On:23 Aug 2022 09:01

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