In situ architecture of the algal nuclear pore complex

Mosalaganti, Shyamal and Kosinski, Jan and Albert, Sahradha and Schaffer, Miroslava and Strenkert, Daniela and Salomé, Patrice A. and Merchant, Sabeeha S. and Plitzko, Jürgen M. and Baumeister, Wolfgang and Engel, Benjamin D. and Beck, Martin. (2018) In situ architecture of the algal nuclear pore complex. Nature Communications, 9 (1). p. 2361.

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Nuclear pore complexes (NPCs) span the nuclear envelope and mediate nucleocytoplasmic exchange. They are a hallmark of eukaryotes and deeply rooted in the evolutionary origin of cellular compartmentalization. NPCs have an elaborate architecture that has been well studied in vertebrates. Whether this architecture is unique or varies significantly in other eukaryotic kingdoms remains unknown, predominantly due to missing in situ structural data. Here, we report the architecture of the algal NPC from the early branching eukaryote Chlamydomonas reinhardtii and compare it to the human NPC. We find that the inner ring of the Chlamydomonas NPC has an unexpectedly large diameter, and the outer rings exhibit an asymmetric oligomeric state that has not been observed or predicted previously. Our study provides evidence that the NPC is subject to substantial structural variation between species. The divergent and conserved features of NPC architecture provide insights into the evolution of the nucleocytoplasmic transport machinery.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology and Biophysics (Engel)
UniBasel Contributors:Engel, Ben
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Publishing Group
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:23 Aug 2022 09:44
Deposited On:23 Aug 2022 09:44

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