In situ structural analysis of Golgi intracisternal protein arrays

Engel, Benjamin D. and Schaffer, Miroslava and Albert, Sahradha and Asano, Shoh and Plitzko, Jürgen M. and Baumeister, Wolfgang. (2015) In situ structural analysis of Golgi intracisternal protein arrays. Proceedings of the National Academy of Sciences of the United States of America, 112 (36). pp. 11264-11269.

PDF - Published Version

Official URL: https://edoc.unibas.ch/89565/

Downloads: Statistics Overview


We acquired molecular-resolution structures of the Golgi within its native cellular environment. Vitreous Chlamydomonas cells were thinned by cryo-focused ion beam milling and then visualized by cryo-electron tomography. These tomograms revealed structures within the Golgi cisternae that have not been seen before. Narrow trans-Golgi lumina were spanned by asymmetric membrane-associated protein arrays that had ∼6-nm lateral periodicity. Subtomogram averaging showed that the arrays may determine the narrow central spacing of the trans-Golgi cisternae through zipper-like interactions, thereby forcing cargo to the trans-Golgi periphery. Additionally, we observed dense granular aggregates within cisternae and intracisternal filament bundles associated with trans-Golgi buds. These native in situ structures provide new molecular insights into Golgi architecture and function.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology and Biophysics (Engel)
UniBasel Contributors:Engel, Ben
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:National Academy of Sciences
Note:Publication type according to Uni Basel Research Database: Journal article
Related URLs:
Identification Number:
edoc DOI:
Last Modified:23 Aug 2022 10:37
Deposited On:23 Aug 2022 10:37

Repository Staff Only: item control page