Zebrafish Larvae as an; in vivo; Model for Antimicrobial Activity Tests against Intracellular; Salmonella;

Hauswirth, Patrick and Buck, Jonas and Puligilla, Ramya and Alter, Claudio Luca and Sieber, Sandro and Claudi, Beatrice and Fanous, Joseph and Bumann, Dirk and Huwyler, Jörg. (2023) Zebrafish Larvae as an; in vivo; Model for Antimicrobial Activity Tests against Intracellular; Salmonella;. Frontiers in bioscience (Landmark edition), 28 (5). p. 99.

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Blood infections from multi-drug-resistant; Salmonella; pose a major health burden. This is especially true because; Salmonella; can survive and replicate intracellularly, and the development of new treatment strategies is dependent on expensive and time-consuming; in vivo; trials. The aim of this study was to develop a; Salmonella; -infection model that makes it possible to directly observe; Salmonella; infections of macrophages; in vivo; and to use this model to test the effect of antimicrobials against intra- and extracellular; Salmonella; in order to close the gap between; in vitro; and rodent-infection models.; We established suitable; Salmonella; -infection conditions using genetically engineered zebrafish and; Salmonella; -expressing fluorescent proteins (; green fluorescent protein; (; GFP; ) and/or; mCherry; ).; We detected; Salmonella; inside and outside zebrafish larvae macrophages. Administration of the cell-impermeable antibiotic tobramycin removed; Salmonella; residing outside macrophages but did not affect; Salmonella; in macrophages, whereas ceftriaxone successfully cleared both types of; Salmonella; .; Salmonella; inside and outside macrophages experienced substantial DNA damage after administration of fluoroquinolones consistent with the excellent cell penetration of these antibiotics.; The zebrafish-larvae model enables testing of antimicrobials for efficacy against extra- and intracellular; Salmonella; in a complex; in vivo; environment. This model thus might serve for antimicrobial lead optimization prior to using rodent models.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Molecular Microbiology (Bumann)
UniBasel Contributors:Bumann, Dirk and Claudi, Beatrice
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:IMR Press
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:29 Nov 2023 16:27
Deposited On:29 Aug 2023 15:47

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