Axis formation in annual killifish: Nodal coordinates morphogenesis in absence of Huluwa prepatterning

Abitua, Philipp B. and Aksel, Deniz C. and Schier, Alexander F.. (2021) Axis formation in annual killifish: Nodal coordinates morphogenesis in absence of Huluwa prepatterning.

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Official URL: https://edoc.unibas.ch/92923/

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Axis formation in fish and amphibians is initiated by a prepattern of maternal gene products in the blastula. The embryogenesis of annual killifish challenges prepatterning models because blastomeres disperse and then re-aggregate to form the germ layers and body axes. This dispersion-aggregation process prompts the question how axis determinants such as Huluwa and germ layer inducers such as Nodal function in annual killifish. Here we show in Nothobranchius furzeri that huluwa, the factor thought to break symmetry by stabilizing β-catenin, is a non-functional pseudogene. Nuclear β-catenin is not selectively stabilized on one side of the blastula but accumulates in cells forming the incipient aggregate. Inhibition of Nodal signaling blocks aggregation and disrupts coordinated cell migration, establishing a novel role for this signaling pathway. These results reveal a surprising departure from classic mechanisms of axis formation: canonical Huluwa-mediated prepatterning is dispensable and Nodal coordinates morphogenesis.
One Sentence Summary Axis formation in annual killifish relies on Nodal to coordinate cell migration and is independent of Huluwa-mediated prepatterning.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell and Developmental Biology (Schier)
UniBasel Contributors:Schier, Alexander F
Item Type:Preprint
Note:Publication type according to Uni Basel Research Database: Discussion paper / Internet publication
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Last Modified:24 Jan 2023 12:09
Deposited On:24 Jan 2023 12:09

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