A new in vitro model to study mitochondria in Parkinson’s Disease

Sironi, Lara. A new in vitro model to study mitochondria in Parkinson’s Disease. 2022, Doctoral Thesis, University of Basel, Faculty of Science.


Official URL: https://edoc.unibas.ch/88836/

Downloads: Statistics Overview


Parkinson’s disease (PD) is a pathogenically complex multifactorial disease and the most common neurodegenerative movement disorder. Several lines of evidence, obtained from studies of familial forms of PD, patient samples, and in vitro/in vivo models, underline a major role of mitochondrial dysfunction, impaired mitochondrial quality control, and dysregulated mitochondrial crosstalk with other organelles.
This project aimed to understand whether moderate overexpression of the inner mitochondrial membrane OPA1 would rescue the mitochondrial dysfunction caused by A53T mutant alpha-synuclein. The underlying rationale for this work was based on the fact that mild overexpression of OPA1 was recently demonstrated to improve the motor performance in addition to biochemical and molecular phenotypes of two mouse models of mitochondrial disease [328]; of note, in mice, mild OPA1 overexpression is compatible with normal development, fertility and lifespan [83]. Therefore, our intention was to cross the Opa1tg mouse with the A53T alpha-synuclein transgenic line M83 model [325] to analyse the mitochondrial phenotype in the double transgenic mouse.
Advisors:Frank, Stephan and Edlich, Frank
Faculties and Departments:03 Faculty of Medicine > Bereich Querschnittsfächer (Klinik) > Pathologie USB > Neuro- und Muskelpathologie (Frank)
03 Faculty of Medicine > Departement Klinische Forschung > Bereich Querschnittsfächer (Klinik) > Pathologie USB > Neuro- und Muskelpathologie (Frank)
05 Faculty of Science > Departement Biozentrum
UniBasel Contributors:Frank, Stephan
Item Type:Thesis
Thesis Subtype:Doctoral Thesis
Thesis no:14755
Thesis status:Complete
Number of Pages:146
Identification Number:
  • urn: urn:nbn:ch:bel-bau-diss147557
edoc DOI:
Last Modified:26 Jul 2022 04:30
Deposited On:25 Jul 2022 14:26

Repository Staff Only: item control page