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The expression of virulence increases outer-membrane permeability and sensitivity to envelope stress in Salmonella Typhimurium

Sobota, Malgorzata and Rodilla Ramirez, Pilar Natalia and Cambré, Alexander and Haas, Tiphaine and Cornillet, Delphine and Rocker, Andrea and Aertsen, Abram and Diard, Médéric. (2021) The expression of virulence increases outer-membrane permeability and sensitivity to envelope stress in Salmonella Typhimurium.

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Abstract

Environmental cues modulate the expression of virulence in bacterial pathogens. However, while cues that upregulate virulence are often intuitive and mechanistically well understood, this is less so for cues that downregulate virulence. In this study, we noticed that upregulation of the HilD virulence regulon in Salmonella Typhimurium ( S .Tm) sensitized cells to membrane stress mediated by cholate, Tris/EDTA or heat. Further monitoring of membrane status and stress resistance of S .Tm cells in relation to virulence expression, revealed that co-expressed virulence factors embedded in the envelope (including the Type Three Secretion System 1 and the flagella) increased permeability, and stress sensitivity of the membrane. Importantly, pretreating the bacteria by sublethal stress inhibited virulence expression and restored stress resistance. As such, these results demonstrate a trade-off between virulence and stress resistance, which explains the downregulation of virulence expression in response to harsh environments in S .Tm.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Pathogen Evolution (Diard)
UniBasel Contributors:Diard, Médéric
Item Type:Working Paper
Publisher:biorxiv.org
Note:Publication type according to Uni Basel Research Database: Discussion paper / Internet publication
Language:English
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Last Modified:08 Mar 2022 09:29
Deposited On:08 Mar 2022 09:29

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