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Miniaturizing EM Sample Preparation: Opportunities, Challenges, and "Visual Proteomics"

Arnold, Stefan A. and Müller, Shirley A. and Schmidli, Claudio and Syntychaki, Anastasia and Rima, Luca and Chami, Mohamed and Stahlberg, Henning and Goldie, Kenneth N. and Braun, Thomas. (2018) Miniaturizing EM Sample Preparation: Opportunities, Challenges, and "Visual Proteomics". Proteomics, 18 (5-6). e1700176.

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Official URL: https://edoc.unibas.ch/78561/

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Abstract

This review compares and discusses conventional versus miniaturized specimen preparation methods for transmission electron microscopy (TEM). The progress brought by direct electron detector cameras, software developments and automation have transformed transmission cryo-electron microscopy (cryo-EM) and made it an invaluable high-resolution structural analysis tool. In contrast, EM specimen preparation has seen very little progress in the last decades and is now one of the main bottlenecks in cryo-EM. Here, we discuss the challenges faced by specimen preparation for single particle EM, highlight current developments, and show the opportunities resulting from the advanced miniaturized and microfluidic sample grid preparation methods described, such as visual proteomics and time-resolved cryo-EM studies.
Faculties and Departments:05 Faculty of Science
05 Faculty of Science > Departement Biozentrum > Services Biozentrum > BioEM Lab (Chami)
UniBasel Contributors:Goldie, Kenneth N. and Chami, Mohamed
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Wiley
ISSN:1615-9853
e-ISSN:1615-9861
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:01 Oct 2020 14:10
Deposited On:01 Oct 2020 07:57

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