Activation mechanism of a small prototypic Rec-GGDEF diguanylate cyclase

Teixeira, Raphael D. and Holzschuh, Fabian and Schirmer, Tilman. (2020) Activation mechanism of a small prototypic Rec-GGDEF diguanylate cyclase.

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Diguanylate cyclases (DGCs) synthesising the bacterial second messenger c-di-GMP are found to be regulated by a variety of sensory input domains that control the activity of their catalytical GGDEF domain. As part of two-component systems, they are activated by cognate histidine kinases that phosphorylate their Rec input domains. DgcR from Leptospira biflexa is a constitutively dimeric prototype of this class of DGCs. Full-length crystal structures revealed that BeF 3 - pseudo-phosphorylation induces a relative rotation of two rigid halves in the Rec domain. This is coupled to a reorganisation of the dimeric structure with concomitant switching of the coiled-coil linker to an alternative heptad register. Finally, the activated register allows the two substrate-loaded GGDEF domains, which are linked to the end of the coiled-coil via a localised hinge, to move into a catalytically competent dimeric arrangement. Bioinformatic analyses suggest that the binary register switch mechanism is utilised by many DGCs with N-terminal coiled-coil linkers.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Schirmer)
UniBasel Contributors:Schirmer, Tilman and Dias Teixeira, Raphael and Holzschuh, Fabian
Item Type:Working Paper
Number of Pages:43
Note:Publication type according to Uni Basel Research Database: Discussion paper / Internet publication
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Last Modified:15 Sep 2020 13:18
Deposited On:15 Sep 2020 13:18

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