Classical Activation of Macrophages Leads to Lipid Droplet Formation Without; de novo; Fatty Acid Synthesis

Rosas-Ballina, Mauricio and Guan, Xue Li and Schmidt, Alexander and Bumann, Dirk. (2020) Classical Activation of Macrophages Leads to Lipid Droplet Formation Without; de novo; Fatty Acid Synthesis. Frontiers in Immunology, 11. p. 131.

[img] PDF - Published Version
Available under License CC BY (Attribution).


Official URL: https://edoc.unibas.ch/76525/

Downloads: Statistics Overview


Altered lipid metabolism in macrophages is associated with various important inflammatory conditions. Although lipid metabolism is an important target for therapeutic intervention, the metabolic requirement involved in lipid accumulation during pro-inflammatory activation of macrophages remains incompletely characterized. We show here that macrophage activation with IFNγ results in increased aerobic glycolysis, iNOS-dependent inhibition of respiration, and accumulation of triacylglycerol. Surprisingly, metabolite tracing with; 13; C-labeled glucose revealed that the glucose contributed to the glycerol groups in triacylglycerol (TAG), rather than to; de novo; synthesis of fatty acids. This is in stark contrast to the otherwise similar metabolism of cancer cells, and previous results obtained in activated macrophages and dendritic cells. Our results establish a novel metabolic pathway whereby glucose provides glycerol to the headgroup of TAG during classical macrophage activation.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Molecular Microbiology (Bumann)
UniBasel Contributors:Bumann, Dirk
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Frontiers Media
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
edoc DOI:
Last Modified:04 May 2020 07:16
Deposited On:04 May 2020 07:16

Repository Staff Only: item control page