Non-specific interference of cobalt with siderophore-dependent iron uptake pathways

Carballido Lopez, Ana and Cunrath, Olivier and Forster, Anne and Pérard, Julien and Graulier, Gwenaëlle and Legendre, Rachel and Varet, Hugo and Sismeiro, Odile and Perraud, Quentin and Pesset, Bénédicte and Saint Auguste, Pamela and Bumann, Dirk and Mislin, Gaëtan L. A. and Coppee, Jean Yves and Michaud-Soret, Isabelle and Fechter, Pierre and Schalk, Isabelle J.. (2019) Non-specific interference of cobalt with siderophore-dependent iron uptake pathways. Metallomics : integrated biometal science, 11 (11). pp. 1937-1951.

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Official URL: https://edoc.unibas.ch/76514/

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Much data shows that biological metals other than Fe; 3+; can interfere with Fe; 3+; acquisition by siderophores in bacteria. Siderophores are small Fe; 3+; chelators produced by the microorganisms to obtain access to Fe; 3+; . Here, we show that Co; 2+; is imported into Pseudomonas aeruginosa cells in a complex with the siderophore pyochelin (PCH) by the ferri-PCH outer membrane transporter FptA. Moreover, the presence of Co; 2+; in the bacterial environment strongly affects the production of PCH. Proteomic and transcriptomic approaches showed that a decrease of PCH production is associated with repression of the expression of the genes involved in PCH biosynthesis. We used various molecular biology approaches to show that this repression is not Fur-(ferric uptake transcriptional regulator) dependent but due to competition of PCH-Co with PCH-Fe for PchR (transcriptional activator), thus inhibiting the formation of PchR-PCH-Fe and consequently the expression of the PCH genes. We observed a similar mechanism of repression of PCH production, but to a lesser extent, by Ni; 2+; , but not for Zn; 2+; , Cu; 2+; , or Mn; 2+; . Here, we show, for the first time at a molecular level, how the presence of a contaminant metal can interfere with Fe; 3+; acquisition by the siderophores PCH and PVD.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Molecular Microbiology (Bumann)
UniBasel Contributors:Bumann, Dirk
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:21 May 2020 08:53
Deposited On:21 May 2020 08:53

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