Giraldez, Antonio J. and Cinalli, Ryan M. and Glasner, Margaret E. and Enright, Anton J. and Thomson, J. Michael and Baskerville, Scott and Hammond, Scott M. and Bartel, David P. and Schier, Alexander F.. (2005) MicroRNAs regulate brain morphogenesis in zebrafish. Science, 308 (5723). pp. 833-838.
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Official URL: https://edoc.unibas.ch/74874/
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Abstract
MicroRNAs (miRNAs) are small RNAs that regulate gene expression posttranscriptionally. To block all miRNA formation in zebrafish, we generated maternal-zygotic dicer (MZdicer) mutants that disrupt the Dicer ribonuclease III and double-stranded RNA-binding domains. Mutant embryos do not process precursor miRNAs into mature miRNAs, but injection of preprocessed miRNAs restores gene silencing, indicating that the disrupted domains are dispensable for later steps in silencing. MZdicer mutants undergo axis formation and differentiate multiple cell types but display abnormal morphogenesis during gastrulation, brain formation, somitogenesis, and heart development. Injection of miR-430 miRNAs rescues the brain defects in MZdicer mutants, revealing essential roles for miRNAs during morphogenesis.
| Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell and Developmental Biology (Schier) |
|---|---|
| UniBasel Contributors: | Schier, Alexander F |
| Item Type: | Article, refereed |
| Article Subtype: | Research Article |
| Publisher: | American Association for the Advancement of Science |
| ISSN: | 0036-8075 |
| e-ISSN: | 1095-9203 |
| Note: | Publication type according to Uni Basel Research Database: Journal article |
| Identification Number: |
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| Last Modified: | 06 Nov 2020 13:13 |
| Deposited On: | 06 Nov 2020 13:13 |
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