MicroRNAs regulate brain morphogenesis in zebrafish

Giraldez, Antonio J. and Cinalli, Ryan M. and Glasner, Margaret E. and Enright, Anton J. and Thomson, J. Michael and Baskerville, Scott and Hammond, Scott M. and Bartel, David P. and Schier, Alexander F.. (2005) MicroRNAs regulate brain morphogenesis in zebrafish. Science, 308 (5723). pp. 833-838.

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Official URL: https://edoc.unibas.ch/74874/

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MicroRNAs (miRNAs) are small RNAs that regulate gene expression posttranscriptionally. To block all miRNA formation in zebrafish, we generated maternal-zygotic dicer (MZdicer) mutants that disrupt the Dicer ribonuclease III and double-stranded RNA-binding domains. Mutant embryos do not process precursor miRNAs into mature miRNAs, but injection of preprocessed miRNAs restores gene silencing, indicating that the disrupted domains are dispensable for later steps in silencing. MZdicer mutants undergo axis formation and differentiate multiple cell types but display abnormal morphogenesis during gastrulation, brain formation, somitogenesis, and heart development. Injection of miR-430 miRNAs rescues the brain defects in MZdicer mutants, revealing essential roles for miRNAs during morphogenesis.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell and Developmental Biology (Schier)
UniBasel Contributors:Schier, Alexander F
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Association for the Advancement of Science
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:06 Nov 2020 13:13
Deposited On:06 Nov 2020 13:13

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