Multicolor Brainbow imaging in zebrafish

Pan, Y. Albert and Livet, Jean and Sanes, Joshua R. and Lichtman, Jeff W. and Schier, Alexander F.. (2011) Multicolor Brainbow imaging in zebrafish. Cold Spring Harbor Protocols, 2011 (1). db.prot5546.

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Official URL: https://edoc.unibas.ch/74720/

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This protocol describes how to use the Brainbow strategy to label neurons in many different hues. The Brainbow system uses a random Cre/lox recombination to create varied combinations of red, blue, and green fluorescent proteins in each cell. The differences in color allow users to follow multiple cells, regardless of how closely they are positioned. This protocol describes how to use Brainbow imaging in zebrafish and provides examples of how to use color as a guide to trace axonal processes. We use the zebrafish trigeminal sensory ganglion as an example and discuss potential modifications for the general use of this technique.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell and Developmental Biology (Schier)
UniBasel Contributors:Schier, Alexander F
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Cold Spring Harbor Laboratory Press
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:23 Nov 2021 16:12
Deposited On:23 Nov 2021 16:12

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