A large-scale zebrafish gene knockout resource for the genome-wide study of gene function

Varshney, Gaurav K. and Lu, Jing and Gildea, Derek E. and Huang, Haigen and Pei, Wuhong and Yang, Zhongan and Huang, Sunny C. and Schoenfeld, David and Pho, Nam H. and Casero, David and Hirase, Takashi and Mosbrook-Davis, Deborah and Zhang, Suiyuan and Jao, Li-En and Zhang, Bo and Woods, Ian G. and Zimmerman, Steven and Schier, Alexander F. and Wolfsberg, Tyra G. and Pellegrini, Matteo and Burgess, Shawn M. and Lin, Shuo. (2013) A large-scale zebrafish gene knockout resource for the genome-wide study of gene function. Genome Research, 23 (4). pp. 727-735.

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Official URL: https://edoc.unibas.ch/74702/

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With the completion of the zebrafish genome sequencing project, it becomes possible to analyze the function of zebrafish genes in a systematic way. The first step in such an analysis is to inactivate each protein-coding gene by targeted or random mutation. Here we describe a streamlined pipeline using proviral insertions coupled with high-throughput sequencing and mapping technologies to widely mutagenize genes in the zebrafish genome. We also report the first 6144 mutagenized and archived F1's predicted to carry up to 3776 mutations in annotated genes. Using in vitro fertilization, we have rescued and characterized ~0.5% of the predicted mutations, showing mutation efficacy and a variety of phenotypes relevant to both developmental processes and human genetic diseases. Mutagenized fish lines are being made freely available to the public through the Zebrafish International Resource Center. These fish lines establish an important milestone for zebrafish genetics research and should greatly facilitate systematic functional studies of the vertebrate genome.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell and Developmental Biology (Schier)
UniBasel Contributors:Schier, Alexander F
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Cold Spring Harbor Laboratory Press
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:23 Nov 2021 16:23
Deposited On:23 Nov 2021 16:23

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