edoc

Precise Timing of Transcription by c-di-GMP Coordinates Cell Cycle and Morphogenesis in Caulobacter

Kaczmarczyk, Andreas and Hempel, Antje M. and von Arx, Christoph and Böhm, Raphael and Dubey, Badri N. and Nesper, Jutta and Schirmer, Tilman and Hiller, Sebastian and Jenal, Urs. (2020) Precise Timing of Transcription by c-di-GMP Coordinates Cell Cycle and Morphogenesis in Caulobacter. Nature Communications, 11 (1). p. 816.

[img]
Preview
PDF - Published Version
Available under License CC BY (Attribution).

3374Kb

Official URL: https://edoc.unibas.ch/74286/

Downloads: Statistics Overview

Abstract

Bacteria adapt their growth rate to their metabolic status and environmental conditions by modulating the length of their G1 period. Here we demonstrate that a gradual increase in the concentration of the second messenger c-di-GMP determines precise gene expression during G1/S transition in Caulobacter crescentus . We show that c-di-GMP stimulates the kinase ShkA by binding to its central pseudo-receiver domain, activates the TacA transcription factor, and initiates a G1/S-specific transcription program leading to cell morphogenesis and S-phase entry. Activation of the ShkA-dependent genetic program causes c-di-GMP to reach peak levels, which triggers S-phase entry and promotes proteolysis of ShkA and TacA. Thus, a gradual increase of c-di-GMP results in precise control of ShkA-TacA activity, enabling G1/S-specific gene expression that coordinates cell cycle and morphogenesis.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Molecular Microbiology (Jenal)
05 Faculty of Science > Departement Biozentrum > Growth & Development > Molecular Microbiology (Jenal)
05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Hiller)
05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Schirmer)
UniBasel Contributors:Schirmer, Tilman and Kaczmarczyk, Andreas and Hempel, Antje Marie and von Arx, Christoph and Böhm, Raphael and Dubey, Badri Nath and Nesper, Jutta and Jenal, Urs and Hiller Odermatt, Sebastian
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Research
e-ISSN:2041-1723
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Related URLs:
Identification Number:
edoc DOI:
Last Modified:17 Jun 2020 06:19
Deposited On:17 Jun 2020 06:19

Repository Staff Only: item control page