Whole-brain activity mapping onto a zebrafish brain atlas

Randlett, Owen and Wee, Caroline L. and Naumann, Eva A. and Nnaemeka, Onyeka and Schoppik, David and Fitzgerald, James E. and Portugues, Ruben and Lacoste, Alix M. B. and Riegler, Clemens and Engert, Florian and Schier, Alexander F.. (2015) Whole-brain activity mapping onto a zebrafish brain atlas. Nature Methods, 12 (11). pp. 1039-1046.

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Official URL: https://edoc.unibas.ch/74123/

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In order to localize the neural circuits involved in generating behaviors, it is necessary to assign activity onto anatomical maps of the nervous system. Using brain registration across hundreds of larval zebrafish, we have built an expandable open-source atlas containing molecular labels and definitions of anatomical regions, the Z-Brain. Using this platform and immunohistochemical detection of phosphorylated extracellular signal-regulated kinase (ERK) as a readout of neural activity, we have developed a system to create and contextualize whole-brain maps of stimulus- and behavior-dependent neural activity. This mitogen-activated protein kinase (MAP)-mapping assay is technically simple, and data analysis is completely automated. Because MAP-mapping is performed on freely swimming fish, it is applicable to studies of nearly any stimulus or behavior. Here we demonstrate our high-throughput approach using pharmacological, visual and noxious stimuli, as well as hunting and feeding. The resultant maps outline hundreds of areas associated with behaviors.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell and Developmental Biology (Schier)
UniBasel Contributors:Schier, Alexander
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:24 May 2020 19:10
Deposited On:24 May 2020 19:10

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