Pharmaceutical development of a lipid-based emulsion containing European Larch (Larix decidua Mill.) oleoresin for the management of chronic wounds

The present work deals with various aspects regarding the oleoresin of the European Larch (Larix decidua), which has been historically documented as a natural remedy used to promote wound healing. Initially, existing knowledge about the chemical composition and therapeutic application of larch oleoresin was consolidated and evaluated through a comprehensive literature review.
In a second step, analytical methods were developed and applied to determine the composition of oleoresins. Such phytochemical identification and analysis methods play an important role in evaluating the composition of plant substances, ranging from chromatographic fingerprints to the quantitative determination of specific compounds. Thus, our goal was to identify the main components of larch oleoresin. The analysis of the chemical composition of the essential oil and the non-volatile fraction of larch oleoresin allowed us to draw conclusions regarding specific quality characteristics such as purity, content, age, and time of extraction. These results can be used as reference data in the characterization of larch oleoresins in the future.
In a third step, a novel pharmaceutical formulation was developed for topical application and for the treatment of chronic wounds. It is a sterile oil-in-water emulsion that can be sprayed onto the skin using an applicator. It has a deodorizing effect, provides a cooling sensation, and supports wound healing. Regarding practical application, stability studies confirmed good chemical and colloidal stability as well as shelf life.
In a fourth step, the new medicinal formulation was produced under the Formula Magistralis, and clinical studies were established. It is our desire to provide health care professionals with additional treatment options for the treatment of chronic wounds. The clinical studies aim to provide initial indications of pharmacological effects and acceptance among patients and medical staff.
Nature provides rich resources for the development of new drugs. Our work demonstrates how the potential of plant-based medicines can be used. The prerequisite is a good understanding of the chemical composition of the ingredients, sensitive analytical methods, suitable dosage forms, and clinical-pharmacological investigations. In the field of traditional phytotherapy, new and therapeutically valuable therapies can be developed.