Biofilm formation on human immune cells is a multicellular predation strategy of Vibrio cholerae

Vidakovic, Lucia and Mikhaleva, Sofya and Jeckel, Hannah and Nisnevich, Valerya and Strenger, Kerstin and Neuhaus, Konstantin and Raveendran, Keerthana and Ben-Moshe, Noa Bossel and Aznaourova, Marina and Nosho, Kazuki and Drescher, Antje and Schmeck, Bernd and Schulte, Leon N. and Persat, Alexandre and Avraham, Roi and Drescher, Knut. (2023) Biofilm formation on human immune cells is a multicellular predation strategy of Vibrio cholerae. Cell, 186 (12). pp. 2690-2704.e20.

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Official URL: https://edoc.unibas.ch/95635/

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Biofilm formation is generally recognized as a bacterial defense mechanism against environmental threats, including antibiotics, bacteriophages, and leukocytes of the human immune system. Here, we show that for the human pathogen Vibrio cholerae, biofilm formation is not only a protective trait but also an aggressive trait to collectively predate different immune cells. We find that V. cholerae forms biofilms on the eukaryotic cell surface using an extracellular matrix comprising primarily mannose-sensitive hemagglutinin pili, toxin-coregulated pili, and the secreted colonization factor TcpF, which differs from the matrix composition of biofilms on other surfaces. These biofilms encase immune cells and establish a high local concentration of a secreted hemolysin to kill the immune cells before the biofilms disperse in a c-di-GMP-dependent manner. Together, these results uncover how bacteria employ biofilm formation as a multicellular strategy to invert the typical relationship between human immune cells as the hunters and bacteria as the hunted.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Microbiology and Biophysics (Drescher)
UniBasel Contributors:Drescher, Knut
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Cell Press
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:11 Sep 2023 10:58
Deposited On:11 Sep 2023 10:58

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