Atomic force microscopy-based mechanobiology

Krieg, Michael and Fläschner, Gotthold and Alsteens, David and Gaub, Benjamin M. and Roos, Wouter H. and Wuite, Gijs J. L. and Gaub, Hermann E. and Gerber, Christoph and Dufrêne, Yves F. and Müller, Daniel J.. (2019) Atomic force microscopy-based mechanobiology. Nature Reviews Physics, 1. pp. 41-57.

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Official URL: https://edoc.unibas.ch/94491/

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Mechanobiology emerges at the crossroads of medicine, biology, biophysics and engineering and describes how the responses of proteins, cells, tissues and organs to mechanical cues contribute to development, differentiation, physiology and disease. The grand challenge in mechanobiology is to quantify how biological systems sense, transduce, respond and apply mechanical signals. Over the past three decades, atomic force microscopy (AFM) has emerged as a key platform enabling the simultaneous morphological and mechanical characterization of living biological systems. In this Review, we survey the basic principles, advantages and limitations of the most common AFM modalities used to map the dynamic mechanical properties of complex biological samples to their morphology. We discuss how mechanical properties can be directly linked to function, which has remained a poorly addressed issue. We outline the potential of combining AFM with complementary techniques, including optical microscopy and spectroscopy of mechanosensitive fluorescent constructs, super-resolution microscopy, the patch clamp technique and the use of microstructured and fluidic devices to characterize the 3D distribution of mechanical responses within biological systems and to track their morphology and functional state.
Faculties and Departments:05 Faculty of Science > Departement Physik > Physik
UniBasel Contributors:Gerber, Christoph
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Research
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:08 May 2023 13:15
Deposited On:08 May 2023 13:15

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