Nuclear localisation is crucial for the proapoptotic activity of the HtrA-like serine protease Nma111p

Belanger, Kenneth D. and Walter, David and Henderson, Tracey A. and Yelton, Allison L. and O'Brien, Travis G. and Belanger, Karyn G. and Geier, Susan J. and Fahrenkrog, Birthe. (2009) Nuclear localisation is crucial for the proapoptotic activity of the HtrA-like serine protease Nma111p. Journal of Cell Science, 122 (21). pp. 3931-3941.

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Programmed cell death is induced by the activation of a subset of intracellular proteins in response to specific extra- and intracellular signals. In the yeast Saccharomyces cerevisiae, Nma111p functions as a nuclear serine protease that is necessary for apoptosis under cellular stress conditions, such as elevated temperature or treatment of cells with hydrogen peroxide to induce cell death. We have examined the role of nuclear protein import in the function of Nma111p in apoptosis. Nma111p contains two small clusters of basic residues towards its N-terminus, both of which are necessary for efficient translocation into the nucleus. Nma111p does not shuttle between the nucleus and cytoplasm during either normal growth conditions or under environmental stresses that induce apoptosis. The N-terminal half of Nma111p is sufficient to provide the apoptosis-inducing activity of the protein, and the nuclear-localisation signal (NLS) sequences and catalytic serine 235 are both necessary for this function. We provide compelling evidence that intranuclear Nma111p activity is necessary for apoptosis in yeast.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Fahrenkrog)
UniBasel Contributors:Fahrenkrog, Birthe and Walter, David
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Company of Biologists
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:27 Mar 2023 10:49
Deposited On:27 Mar 2023 10:49

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