Characterization of RNA content in individual phase-separated coacervate microdroplets

Wollny, Damian and Vernot, Benjamin and Wang, Jie and Hondele, Maria and Safrastyan, Aram and Aron, Franziska and Micheel, Julia and He, Zhisong and Hyman, Anthony and Weis, Karsten and Camp, J. Gray and Tang, T.-Y Dora and Treutlein, Barbara. (2022) Characterization of RNA content in individual phase-separated coacervate microdroplets. Nature communications, 13 (1). p. 2626.

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Condensates formed by complex coacervation are hypothesized to have played a crucial part during the origin-of-life. In living cells, condensation organizes biomolecules into a wide range of membraneless compartments. Although RNA is a key component of biological condensates and the central component of the RNA world hypothesis, little is known about what determines RNA accumulation in condensates and to which extend single condensates differ in their RNA composition. To address this, we developed an approach to read the RNA content from single synthetic and protein-based condensates using high-throughput sequencing. We find that certain RNAs efficiently accumulate in condensates. These RNAs are strongly enriched in sequence motifs which show high sequence similarity to short interspersed elements (SINEs). We observe similar results for protein-derived condensates, demonstrating applicability across different in vitro reconstituted membraneless organelles. Thus, our results provide a new inroad to explore the RNA content of phase-separated droplets at single condensate resolution.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Biochemistry (Hondele)
UniBasel Contributors:Hondele, Maria
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:23 Oct 2023 13:29
Deposited On:21 Feb 2023 13:06

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