A fluorescent perilipin 2 knock-in mouse model visualizes lipid droplets in the developing and adult brain

Madsen, S. and Delgado, A. C. and Cadilhac, C. and Battison, F. and Maillard, V. and Magrinelli, E. and Jabaudon, D. and Telley, T. and Doetsch, F. and Knobloch, M.. (2022) A fluorescent perilipin 2 knock-in mouse model visualizes lipid droplets in the developing and adult brain.

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Official URL: https://edoc.unibas.ch/91765/

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Lipid droplets (LDs) are dynamic lipid storage organelles. They are tightly linked to metabolism and can exert protective functions, making them important players in health and disease. Most LD studies in vivo rely on staining methods, providing only a snapshot. We therefore developed a LD-reporter mouse by endogenously labelling the LD coat protein perilipin 2 (PLIN2) with tdTomato, enabling staining-free fluorescent LD visualisation in living and fixed tissues and cells. Here we validate this model under standard and high-fat diet conditions and demonstrate that LDs are present in various cells in the healthy brain, including neurons, astrocytes, ependymal cells, neural stem/progenitor cells and microglia. Furthermore, we show that LDs are abundant during brain development and can be visualized using live-imaging of embryonic slices. Taken together, our tdTom-Plin2 mouse serves as a novel tool to study LDs and their dynamics under both physiological and diseased conditions in all tissues expressing Plin2.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Neurobiology > Stem Cell Biology (Doetsch)
UniBasel Contributors:Doetsch, Fiona and Delgado Fumero, Ana
Item Type:Preprint
Note:Publication type according to Uni Basel Research Database: Discussion paper / Internet publication
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Last Modified:21 Dec 2022 08:16
Deposited On:21 Dec 2022 08:16

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