A DNA-micropatterned surface for propagating biomolecular signals by positional on-off assembly of catalytic nanocompartments

Maffeis, Vivivana and Hürlimann, Dimitri and Krywko-Cendrowska, Agata and Schönenberger, Cora-Ann and Housecroft, Catherine E. and Palivan, Cornelia G.. (2022) A DNA-micropatterned surface for propagating biomolecular signals by positional on-off assembly of catalytic nanocompartments. Small, 19 (13, Special Issue: Synthetic Biology and Biomimicry). p. 2202818.

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Signal transduction is pivotal for the transfer of information between and within living cells. The composition and spatial organization of specified compartments are key to propagating soluble signals. Here, a high-throughput platform mimicking multistep signal transduction which is based on a geometrically defined array of immobilized catalytic nanocompartments (CNCs) that consist of distinct polymeric nanoassemblies encapsulating enzymes and DNA or enzymes alone is presented. The dual role of single entities or tandem CNCs in providing confined but communicating spaces for complex metabolic reactions and in protecting encapsulated compounds from denaturation is explored. To support a controlled spatial organization of CNCs, CNCs are patterned by means of DNA hybridization to a microprinted glass surface. Specifically, CNC-functionalized DNA microarrays are produced where individual reaction compartments are kept in close proximity by a distinct geometrical arrangement to promote effective communication. Besides a remarkable versatility and robustness, the most prominent feature of this platform is the reversibility of DNA-mediated CNC-anchoring which renders it reusable. Micropatterns of polymer-based nanocompartment assemblies offer an ideal scaffold for the development of the next generation responsive and communicative soft-matter analytical devices for applications in catalysis and medicine.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Former Organization Units Chemistry > Anorganische Chemie (Housecroft)
05 Faculty of Science > Departement Chemie > Chemie > Physikalische Chemie (Palivan)
UniBasel Contributors:Housecroft, Catherine Elizabeth and Maffeis, Viviana and Krywko-Cendrowska, Agata Nora and Schönenberger, Cora-Ann and Palivan, Cornelia G and Hürlimann, Dimitri
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:03 Apr 2023 11:47
Deposited On:03 Apr 2023 08:27

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