Stein, Alina and Liang, Alexandria Deliz and Sahin, Reyhan and Ward, Thomas R.. (2022) Incorporation of metal-chelating unnatural amino acids into halotag for allylic deamination. Journal of Organometallic Chemistry, 962. p. 122272.
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Abstract
The potential of artificial metalloenzymes has led to an increase in interest for the design of novel metal-binding sites in proteins. Metal-chelating unnatural amino acids offer an auspicious solution to engineer active metal sites in a defined way. Herein, we describe the introduction of four metal-chelating unnatural amino acids into HaloTag, an attractive scaffold for the assembly of functional artificial metalloenzymes. HaloTag, engineered with 2-amino-3-(8-hydroxyquinolin-5-yl)propanoic acid (HQ-Ala-1) was used to assemble an artificial metalloenzyme for improved allylic deamination upon complementation with [(η5-C5H5)Ru(MeCN)3]+.
Faculties and Departments: | 05 Faculty of Science > Departement Chemie > Chemie > Bioanorganische Chemie (Ward) |
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UniBasel Contributors: | Ward, Thomas R. R. |
Item Type: | Article, refereed |
Article Subtype: | Research Article |
Publisher: | Elsevier |
ISSN: | 0022-328X |
Note: | Publication type according to Uni Basel Research Database: Journal article |
Language: | English |
Identification Number: | |
edoc DOI: | |
Last Modified: | 14 Feb 2022 15:51 |
Deposited On: | 14 Feb 2022 15:51 |
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