Gallaud, Emmanuel and Ramdas Nair, Anjana and Horsley, Nicole and Monnard, Arnaud and Singh, Priyanka and Pham, Tri Thanh and Salvador Garcia, David and Ferrand, Alexia and Cabernard, Clemens. (2020) Dynamic centriolar localization of Polo and Centrobin in early mitosis primes centrosome asymmetry. PLoS Biology, 18 (8). e3000762.
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Official URL: https://edoc.unibas.ch/86658/
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Abstract
Centrosomes, the main microtubule organizing centers (MTOCs) of metazoan cells, contain an older "mother" and a younger "daughter" centriole. Stem cells either inherit the mother or daughter-centriole-containing centrosome, providing a possible mechanism for biased delivery of cell fate determinants. However, the mechanisms regulating centrosome asymmetry and biased centrosome segregation are unclear. Using 3D-structured illumination microscopy (3D-SIM) and live-cell imaging, we show in fly neural stem cells (neuroblasts) that the mitotic kinase Polo and its centriolar protein substrate Centrobin (Cnb) accumulate on the daughter centriole during mitosis, thereby generating molecularly distinct mother and daughter centrioles before interphase. Cnb's asymmetric localization, potentially involving a direct relocalization mechanism, is regulated by Polo-mediated phosphorylation, whereas Polo's daughter centriole enrichment requires both Wdr62 and Cnb. Based on optogenetic protein mislocalization experiments, we propose that the establishment of centriole asymmetry in mitosis primes biased interphase MTOC activity, necessary for correct spindle orientation.
Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum 05 Faculty of Science > Departement Biozentrum > Services Biozentrum > Imaging Core Facility (Biehlmaier) |
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UniBasel Contributors: | Loynton-Ferrand, Alexia |
Item Type: | Article, refereed |
Article Subtype: | Research Article |
Publisher: | Public Library of Science |
ISSN: | 1544-9173 |
e-ISSN: | 1545-7885 |
Note: | Publication type according to Uni Basel Research Database: Journal article |
Language: | English |
Identification Number: |
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edoc DOI: | |
Last Modified: | 31 Jan 2022 11:54 |
Deposited On: | 31 Jan 2022 11:54 |
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