Sawh, Ahilya N. and Mango, Susan E.. (2020) Multiplexed Sequential DNA FISH in Caenorhabditis elegans Embryos. STAR Protocols, 1 (3). p. 100107.
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Abstract
This protocol describes a high-throughput and multiplexed DNA fluorescence; in situ; hybridization method to trace chromosome conformation in; Caenorhabditis elegans; embryos. This approach generates single-cell and single-chromosome localization data that can be used to determine chromosome conformation and assess the heterogeneity of structures that exist; in vivo; . This strategy is flexible through modifications to the probe design steps to interrogate chromosome structure at the desired genomic scale (small-scale loops to whole-chromosome organization). For complete details on the use and execution of this protocol, please refer to Sawh et al. (2020).
Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum 05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell and Developmental Biology (Mango) |
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UniBasel Contributors: | Mango, Susan Elizabeth and Sawh, Ahilya Nirvana |
Item Type: | Article, refereed |
Article Subtype: | Research Article |
Publisher: | Cell Press |
e-ISSN: | 2666-1667 |
Note: | Publication type according to Uni Basel Research Database: Journal article |
Language: | English |
Identification Number: |
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edoc DOI: | |
Last Modified: | 27 Apr 2023 09:55 |
Deposited On: | 07 Sep 2021 10:40 |
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