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Multiplexed Sequential DNA FISH in Caenorhabditis elegans Embryos

Sawh, Ahilya N. and Mango, Susan E.. (2020) Multiplexed Sequential DNA FISH in Caenorhabditis elegans Embryos. STAR Protocols, 1 (3). p. 100107.

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Abstract

This protocol describes a high-throughput and multiplexed DNA fluorescence; in situ; hybridization method to trace chromosome conformation in; Caenorhabditis elegans; embryos. This approach generates single-cell and single-chromosome localization data that can be used to determine chromosome conformation and assess the heterogeneity of structures that exist; in vivo; . This strategy is flexible through modifications to the probe design steps to interrogate chromosome structure at the desired genomic scale (small-scale loops to whole-chromosome organization). For complete details on the use and execution of this protocol, please refer to Sawh et al. (2020).
Faculties and Departments:05 Faculty of Science > Departement Biozentrum
05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell and Developmental Biology (Mango)
UniBasel Contributors:Mango, Susan Elizabeth and Sawh, Ahilya Nirvana
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Cell Press
e-ISSN:2666-1667
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
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edoc DOI:
Last Modified:07 Sep 2021 10:40
Deposited On:07 Sep 2021 10:40

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