Kemmerling, Simon and Ziegler, Jörg and Schweighauser, Gabriel and Arnold, Stefan A. and Giss, Dominic and Müller, Shirley A. and Ringler, Philippe and Goldie, Kenneth N. and Goedecke, Nils and Hierlemann, Andreas and Stahlberg, Henning and Engel, Andreas and Braun, Thomas. (2012) Connecting μ-fluidics to electron microscopy. Journal of Structural Biology, 177 (1). pp. 128-134.
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Official URL: https://edoc.unibas.ch/83874/
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Abstract
A versatile methodology for electron microscopy (EM) grid preparation enabling total content sample analysis is presented. A microfluidic-dialysis conditioning module to desalt or mix samples with negative stain solution is used, combined with a robotic writing table to micro-pattern the EM grids. The method allows heterogeneous samples of minute volumes to be processed at physiological pH for structure and mass analysis, and allows the preparation characteristics to be finely tuned.
| Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Stahlberg) 05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Braun) |
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| UniBasel Contributors: | Braun, Thomas and Müller, Shirley and Ringler, Philippe and Goldie, Kenneth N. and Stahlberg, Henning and Engel, Andreas H |
| Item Type: | Article, refereed |
| Article Subtype: | Research Article |
| Publisher: | Elsevier |
| ISSN: | 1047-8477 |
| e-ISSN: | 1095-8657 |
| Note: | Publication type according to Uni Basel Research Database: Journal article |
| Related URLs: | |
| Identification Number: |
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| Last Modified: | 11 Oct 2023 09:14 |
| Deposited On: | 06 Oct 2021 11:49 |
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