Breakdown of Vibrio cholerae biofilm architecture induced by antibiotics disrupts community barrier function

Díaz-Pascual, Francisco and Hartmann, Raimo and Lempp, Martin and Vidakovic, Lucia and Song, Boya and Jeckel, Hannah and Thormann, Kai M. and Yildiz, Fitnat H. and Dunkel, Jörn and Link, Hannes and Nadell, Carey D. and Drescher, Knut. (2019) Breakdown of Vibrio cholerae biofilm architecture induced by antibiotics disrupts community barrier function. Nature Microbiology, 4 (12). pp. 2136-2145.

[img] PDF - Accepted Version

Official URL: https://edoc.unibas.ch/83592/

Downloads: Statistics Overview


Bacterial cells in nature are frequently exposed to changes in their chemical environment; 1,2; . The response mechanisms of isolated cells to such stimuli have been investigated in great detail. By contrast, little is known about the emergent multicellular responses to environmental changes, such as antibiotic exposure; 3-7; , which may hold the key to understanding the structure and functions of the most common type of bacterial communities: biofilms. Here, by monitoring all individual cells in Vibrio cholerae biofilms during exposure to antibiotics that are commonly administered for cholera infections, we found that translational inhibitors cause strong effects on cell size and shape, as well as biofilm architectural properties. We identified that single-cell-level responses result from the metabolic consequences of inhibition of protein synthesis and that the community-level responses result from an interplay of matrix composition, matrix dissociation and mechanical interactions between cells. We further observed that the antibiotic-induced changes in biofilm architecture have substantial effects on biofilm population dynamics and community assembly by enabling invasion of biofilms by bacteriophages and intruder cells of different species. These mechanistic causes and ecological consequences of biofilm exposure to antibiotics are an important step towards understanding collective bacterial responses to environmental changes, with implications for the effects of antimicrobial therapy on the ecological succession of biofilm communities.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Microbiology and Biophysics (Drescher)
UniBasel Contributors:Drescher, Knut
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Publishing Group
Note:Publication type according to Uni Basel Research Database: Journal article
Related URLs:
Identification Number:
edoc DOI:
Last Modified:25 Jun 2021 08:41
Deposited On:25 Jun 2021 08:41

Repository Staff Only: item control page