Droplet Microfluidics and Directed Evolution of Enzymes: an Intertwined Journey

Stucki, Ariane and Vallapurackal, Jaicy and Ward, Thomas R. and Dittrich, Petra S.. (2021) Droplet Microfluidics and Directed Evolution of Enzymes: an Intertwined Journey. Angewandte Chemie International Edition. n/a.

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Evolution is essential to the appearance of complexity and ultimately Life. It relies on the propagation of the properties, traits and characteristics that allow an organism to survive in a challenging environment. It is evolution that shaped our world over about four billion years by slow and iterative adaptation. While natural evolution based on selection is slow and gradual, directed evolution allows the fast and streamlined optimization of a phenotype under selective conditions. The potential of directed evolution for the discovery and optimization of enzymes is mostly limited by the throughput of the tools and methods available for screening. Over the past twenty years, versatile tools based on droplet microfluidics have been developed to address the need for higher throughput. In this review, we provide a chronological overview of the intertwined development of microfluidics droplet-based compartmentalization methods and in vivo directed evolution of enzymes.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Bioanorganische Chemie (Ward)
UniBasel Contributors:Ward, Thomas R. R.
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:27 May 2021 10:30
Deposited On:27 May 2021 10:30

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