Functional and Structural Insights into a Novel Promiscuous Ketoreductase of the Lugdunomycin Biosynthetic Pathway

Xiao, Xiansha and Elsayed, Somayah S. and Wu, Changsheng and van der Heul, Helga U. and Metsä-Ketelä, Mikko and Du, Chao and Prota, Andrea E. and Chen, Chun-Chi and Liu, Weidong and Guo, Rey-Ting and Abrahams, Jan Pieter and van Wezel, Gilles P.. (2020) Functional and Structural Insights into a Novel Promiscuous Ketoreductase of the Lugdunomycin Biosynthetic Pathway. ACS Chemical Biology, 15 (9). pp. 2529-2538.

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Angucyclines are a structurally diverse class of actinobacterial natural products defined by their varied polycyclic ring systems, which display a wide range of biological activities. We recently discovered lugdunomycin (; 1; ), a highly rearranged polyketide antibiotic derived from the angucycline backbone that is synthesized via several yet unexplained enzymatic reactions. Here, we show via; in vivo; ,; in vitro; , and structural analysis that the promiscuous reductase LugOII catalyzes both a C6 and an unprecedented C1 ketoreduction. This then sets the stage for the subsequent C-ring cleavage that is key to the rearranged scaffolds of; 1; . The 1.1 Å structures of LugOII in complex with either ligand 8-; O; -Methylrabelomycin (; 4; ) or 8-; O; -Methyltetrangomycin (; 5; ) and of apoenzyme were resolved, which revealed a canonical Rossman fold and a remarkable conformational change during substrate capture and release. Mutational analysis uncovered key residues for substrate access, position, and catalysis as well as specific determinants that control its dual functionality. The insights obtained in this work hold promise for the discovery and engineering of other promiscuous reductases that may be harnessed for the generation of novel biocatalysts for chemoenzymatic applications.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Nano-diffraction of Biological Specimen (Abrahams)
UniBasel Contributors:Abrahams, Jan Pieter
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Chemical Society
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:20 Apr 2021 12:13
Deposited On:20 Apr 2021 12:13

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