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Modular instrumentation for capillary electrophoresis with laser induced fluorescence detection using plug-and-play microfluidic, electrophoretic and optic modules

Lienard-Mayor, Theo and Furter, Jasmine S. and Taverna, Myriam and Pham, Hung Viet and Hauser, Peter C. and Mai, Thanh Duc. (2020) Modular instrumentation for capillary electrophoresis with laser induced fluorescence detection using plug-and-play microfluidic, electrophoretic and optic modules. Analytica Chimica Acta, 1135. pp. 47-54.

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Official URL: https://edoc.unibas.ch/80566/

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Abstract

This study reports on the development of a novel instrument for capillary electrophoresis (CE) coupled with laser induced fluorescence (LIF) detection that is inspired by the Lego-toy concept. The Lego CE-LIF design is an evolution of purpose-made CE instrumentation, allowing the users to construct their own analytical device with a high degree of standardization (i.e. a "standard" setup) without requirement of mechanical and electronic workshop facilities. To allow instrument reproduction outside the original fabrication laboratory, which is not trivial for in-house-built CE systems, the new design is based on unprecedent 'plugging' hyphenation of various off-the-shelf parts available for microfluidics, optics and electrophoresis. To render the operation with Lego CE-LIF optimal, we developed a new background electrolyte (BGE), using for the first time extremely high concentrations of zwitterionic and large weakly charged species for much improvement of detection sensitivity. The Lego CE-LIF was demonstrated for separation and detection of oligosaccharides labelled with 8-aminopyrene-1,3,6-trisulfonic acid (APTS). The new gel-free BGE for oligosaccharide analysis also allowed simplification of the conventional CE-LIF protocol used with commercial instruments while keeping satisfactory separation performances. Furthermore, the new BGE is fully compatible with a non-thermostatted Lego CE instrument thanks to low current and therefore low heat generation under application of a high voltage. (C) 2020 Elsevier B.V. All rights reserved.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Analytische Chemie (Hauser)
UniBasel Contributors:Hauser, Peter C. P. C.
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Elsevier
ISSN:0003-2670
e-ISSN:1873-4324
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:16 Feb 2021 10:34
Deposited On:16 Feb 2021 10:28

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