edoc

Multicompartment Polymer Vesicles with Artificial Organelles for Signal-Triggered Cascade Reactions Including Cytoskeleton Formation

Belluati, Andrea and Thamboo, Sagana and Najer, Adrian and Maffeis, Viviana and von Planta, Claudio and Craciun, Ioana and Palivan, Cornelia G. and Meier, Wolfgang. (2020) Multicompartment Polymer Vesicles with Artificial Organelles for Signal-Triggered Cascade Reactions Including Cytoskeleton Formation. Advanced Functional Materials, 30 (32). p. 2002949.

[img]
Preview
PDF - Published Version
Available under License CC BY (Attribution).

5Mb

Official URL: https://edoc.unibas.ch/79774/

Downloads: Statistics Overview

Abstract

Abstract Organelles, i.e., internal subcompartments of cells, are fundamental to spatially separate cellular processes, while controlled intercompartment communication is essential for signal transduction. Furthermore, dynamic remodeling of the cytoskeleton provides the mechanical basis for cell shape transformations and mobility. In a quest to develop cell-like smart synthetic materials, exhibiting functional flexibility, a self-assembled vesicular multicompartment system, comprised of a polymeric membrane (giant unilamellar vesicle, GUV) enveloping polymeric artificial organelles (vesicles, nanoparticles), is herein presented. Such multicompartment assemblies respond to an external stimulus that is transduced through a precise sequence. Stimuli-triggered communication between two types of internal artificial organelles induces and localizes an enzymatic reaction and allows ion-channel mediated release from storage vacuoles. Moreover, cytoskeleton formation in the GUVs` lumen can be triggered by addition of ionophores and ions. An additional level of control is achieved by signal-triggered ionophore translocation from organelles to the outer membrane, triggering cytoskeleton formation. This system is further used to study the diffusion of various cytoskeletal drugs across the synthetic outer membrane, demonstrating potential applicability, e.g., anticancer drug screening. Such multicompartment assemblies represent a robust system harboring many different functionalities and are a considerable leap in the application of cell logics to reactive and smart synthetic materials.
Faculties and Departments:05 Faculty of Science > Departement Chemie
05 Faculty of Science > Departement Chemie > Chemie > Makromolekulare Chemie (Meier)
UniBasel Contributors:Meier, Wolfgang P.
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Wiley
ISSN:1616-301X
e-ISSN:1616-3028
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Related URLs:
Identification Number:
edoc DOI:
Last Modified:03 Mar 2021 11:36
Deposited On:10 Feb 2021 11:33

Repository Staff Only: item control page