A review of methods to determine viability, vitality and metabolic rates in microbiology

Braissant, Olivier and Astasov-Frauenhoffer, Monika and Waltimo, Tuomas and Bonkat, Gernot. (2020) A review of methods to determine viability, vitality and metabolic rates in microbiology. Frontiers in Microbiology, 11. p. 547458.

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Official URL: https://edoc.unibas.ch/79293/

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Viability and metabolic assays are commonly used as proxies to assess the overall metabolism of microorganisms. The variety of these assays combined with little information provided by some assay kits or online protocols often leads to mistakes or poor interpretation of the results. In addition, the use of some of these assays is restricted to simple systems (mostly pure cultures), and care must be taken in their application to environmental samples. In this review, the necessary data are compiled to understand the reactions or measurements performed in many of the assays commonly used in various aspects of microbiology. Also, their relationships to each other, as metabolism links many of these assays, resulting in correlations between measured values and parameters, are discussed. Finally, the limitations of these assays are discussed.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedical Engineering
UniBasel Contributors:Braissant, Olivier
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Frontiers Media
Note:Publication type according to Uni Basel Research Database: Journal article
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edoc DOI:
Last Modified:23 Nov 2020 08:34
Deposited On:23 Nov 2020 08:34

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