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Amyloid-like aggregation of provasopressin

Spiess, Martin and Beuret, Nicole and Prescianotto Baschong, Cristina and Rutishauser, Jonas. (2020) Amyloid-like aggregation of provasopressin. Vitamins and hormones, 113. pp. 55-77.

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Official URL: https://edoc.unibas.ch/78266/

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Abstract

The antidiuretic hormone vasopressin is synthesized as a longer precursor protein. After folding in the endoplasmic reticulum (ER), provasopressin is transported through the secretory pathway, forms secretory granules in the trans-Golgi network (TGN), is processed, and finally secreted into the circulation. Mutations in provasopressin cause autosomal dominant diabetes insipidus. They prevent native protein folding and cause fibrillar, amyloid-like aggregation in the ER, which eventually results in cell death. Secretory granules of peptide hormones were proposed to constitute functional amyloids and thus might be the cause of amyloid formation of misfolded mutant protein in the ER. Indeed, the same two segments in the precursor-vasopressin and a C-terminal glycopeptide-were found to be responsible for pathological aggregation in the ER and physiological aggregation in granule formation in the TGN. Furthermore, even wild-type provasopressin tends to aggregate in the ER, but is controlled by ER-associated degradation. When essential components thereof, Sel1L or Hrd1, were inactivated, wild-type provasopressin accumulated as fibrillar aggregates in vasopressinergic neurons in mice, causing diabetes insipidus. Evolution of amyloidogenic sequences for granule formation thus made provasopressin dependent on ER quality control mechanisms. These principles may similarly apply to other peptide hormones.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Biochemistry (Spiess)
UniBasel Contributors:Spiess, Martin and Beuret, Nicole and Baschong, Cristina and Rutishauser, Jonas
Item Type:Article, refereed
Article Subtype:Research Article
ISSN:0083-6729
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:24 Nov 2021 16:55
Deposited On:24 Nov 2021 16:55

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