Insight into the assembly of viruses with vertical single β-barrel major capsid proteins

Gil-Carton, David and Abrescia, Nicola GA and Charro, Diego and Peralta, Bibiana and Castano-Diez, Daniel and Oksanen, Hanna M. and Bamford, Dennis H. and Jaakkola, Salla T. . (2015) Insight into the assembly of viruses with vertical single β-barrel major capsid proteins. Structure, 23 (10). pp. 1866-1877.

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Official URL: https://edoc.unibas.ch/77903/

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Archaeal viruses constitute the least explored niche within the virosphere. Structure-based approaches have revealed close relationships between viruses infecting organisms from different domains of life. Here, using biochemical and cryo-electron microscopy techniques, we solved the structure of euryarchaeal, halophilic, internal membrane-containing Haloarcula hispanica icosahedral virus 2 (HHIV-2). We show that the density of the two major capsid proteins (MCPs) recapitulates vertical single β-barrel proteins and that disulfide bridges stabilize the capsid. Below, ordered density is visible close to the membrane and at the five-fold vertices underneath the host-interacting vertex complex underpinning membrane-protein interactions. The HHIV-2 structure exemplifies the division of conserved architectural elements of a virion, such as the capsid, from those that evolve rapidly due to selective environmental pressure such as host-recognizing structures. We propose that in viruses with two vertical single β-barrel MCPs the vesicle is indispensable, and membrane-protein interactions serve as protein-railings for guiding the assembly.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum
UniBasel Contributors:Castano Diez, Daniel
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Cell Press
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:21 Aug 2020 12:44
Deposited On:21 Aug 2020 12:44

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