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Molecular malaria surveillance using a novel protocol for extraction and analysis of nucleic acids retained on used rapid diagnostic tests

Guirou, Etienne and Schindler, Tobias and Hosch, Salome and Donfack, Olivier Tresor and Yoboue, Charlene Aya and Kraehenbuehl, Silvan and Deal, Anna and Cosi, Glenda and Gondwe, Linda and Mwangoka, Grace and Masuki, Heavenlight and Salim, Nahya and Mpina, Maxmillian and Said, Jongo and Abdulla, Salim and Hoffman, Stephen and Nlavo, Bonifacio Manguire and Maas, Carl and Falla, Carlos Cortes and Phiri, Wonder and Garcia, Guillermo and Tanner, Marcel and Daubenberger, Claudia. (2020) Molecular malaria surveillance using a novel protocol for extraction and analysis of nucleic acids retained on used rapid diagnostic tests. Scientific Reports. London.

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Abstract

The use of malaria rapid diagnostic tests (RDTs) as a source for nucleic acids that can be analyzed via nucleic acid amplification techniques has several advantages, including minimal amounts of blood, sample collection, simplified storage and shipping conditions at room temperature. We have systematically developed and extensively evaluated a procedure to extract total nucleic acids from used malaria RDTs. The co-extraction of DNA and RNA molecules from small volumes of dried blood retained on the RDTs allows detection and quantification of P. falciparum parasites from asymptomatic patients with parasite densities as low as 1 Pf/µL blood using reverse transcription quantitative PCR. Based on the extraction protocol we have developed the ENAR (Extraction of Nucleic Acids from RDTs) approach; a complete workflow for large-scale molecular malaria surveillance. Using RDTs collected during a malaria indicator survey we demonstrated that ENAR provides a powerful tool to analyze nucleic acids from thousands of RDTs in a standardized and high-throughput manner. We found several, known and new, non-synonymous single nucleotide polymorphisms in the propeller region of the kelch 13 gene among isolates circulating on Bioko Island, Equatorial Guinea.
Faculties and Departments:09 Associated Institutions
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology (MPI)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology (MPI) > Clinical Immunology (Daubenberger)
03 Faculty of Medicine > Departement Public Health > Sozial- und Präventivmedizin > Malaria Vaccines (Tanner)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Epidemiology and Public Health (EPH) > Health Interventions > Malaria Vaccines (Tanner)
UniBasel Contributors:Schindler, Tobias and Guirou, Etienne and Hosch, Salome and Yoboue, Aya Charlene and Deal, Anna and Mpina, Maximillian and Tanner, Marcel and Daubenberger, Claudia
Item Type:Other
Publisher:Scientific Reports
ISSN:2045-2322
Note:Publication type according to Uni Basel Research Database: Other publications
Language:English
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Last Modified:05 Sep 2020 03:10
Deposited On:27 Jul 2020 08:10

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