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Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya

Akoko, James and Pelle, Roger and Kivali, Velma and Schelling, Esther and Shirima, Gabriel and Machuka, Eunice M. and Mathew, Coletha and Fèvre, Eric M. and Kyallo, Victoria and Falzon, Laura C. and Lukambagire, AbdulHamid S. and Halliday, Jo E. B. and Bonfoh, Bassirou and Kazwala, Rudovick and Ouma, Collins. (2020) Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya. BMC veterinary research, 16. p. 133.

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Abstract

Brucellosis is an emerging yet neglected zoonosis that has been reported in Kenya. Epidemiological data on brucellosis in ruminants is readily accessible; however, reports on brucellosis in pigs remain limited. This study sought to detect Brucella infection in pig serum by both serological and molecular techniques. Serum from 700 pigs randomly collected at a centralized abattoir in Nairobi region, Kenya were screened in parallel, using both Rose Bengal Test (RBT) and competitive Enzyme-Linked Immuno-sorbent Assay (cELISA) for antibodies against Brucella spp. All sera positive by RBT and 16 randomly selected negative samples were further tested using conventional PCR targeting bcsp31 gene and real-time PCR (RT-PCR) assays targeting IS711 and bcsp31 genes.; A prevalence of 0.57% (n = 4/700) was estimated using RBT; none of these samples was positive on cELISA. All RBT positive sera were also positive by both PCRs, while two sero-negative samples also tested positive on RT-PCR (n = 6/20). Brucella abortus was detected in four out of the six PCR positive samples through a real-time multiplex PCR.; The detection of antibodies against Brucella spp. and DNA in serum from slaughterhouse pigs confirm the presence of Brucella in pigs. Therefore, investigation of the epidemiology and role of pigs in the transmission of brucellosis in Kenya is needed. Further targeted studies would be useful to systematically quantify and identify the spp. of Brucella in pigs.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Epidemiology and Public Health (EPH) > Human and Animal Health > Mobile Populations and Health (Schelling)
UniBasel Contributors:Schelling, Esther
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:BioMed Central
ISSN:1746-6148
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:14 May 2020 08:52
Deposited On:14 May 2020 08:52

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