2VQ1: Anti Trimeric Lewis X Fab54-5C10-A

De Geus, D. C. and Van Roon, A. M. M. and Thomassen, E. A. J. and Hokke, C. H. and Deelder, A. M. and Abrahams, J. P.. (2009) 2VQ1: Anti Trimeric Lewis X Fab54-5C10-A. Worldwide Protein Data Bank. 2VQ1.

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Official URL: https://edoc.unibas.ch/75929/

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Lewis X trisaccharides normally function as essential cell-cell interaction mediators. However, oligomers of Lewis X trisaccharides expressed by the parasite Schistosoma mansoni seem to be related to its evasion of the immune response of its human host. Here we show that monoclonal antibody 54-5C10-A, which is used to diagnose schistosomiasis in humans, interacts with oligomers of at least three Lewis X trisaccharides, but not with monomeric Lewis X. We describe the sequence and the 2.5 A crystal structure of its Fab fragment and infer a possible mode of binding of the polymeric Lewis X from docking studies. Our studies indicate a radically different mode of binding compared to Fab 291-2G3-A, which is specific for monomeric Lewis X, thus providing a structural explanation of the diagnostic success of 54-5C10-A.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Nano-diffraction of Biological Specimen (Abrahams)
UniBasel Contributors:Abrahams, Jan Pieter
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:24 Nov 2021 10:34
Deposited On:24 Nov 2021 10:34

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