Imaging protein three-dimensional nanocrystals with cryo-EM

Nederlof, Igor and Li, Yao Wang and van Heel, Marin and Abrahams, Jan Pieter. (2013) Imaging protein three-dimensional nanocrystals with cryo-EM. Acta Crystallographica. Section D, Biological Crystallography, 69. pp. 852-859.

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Flash-cooled three-dimensional crystals of the small protein lysozyme with a thickness of the order of 100 nm were imaged by 300 kV cryo-EM on a Falcon direct electron detector. The images were taken close to focus and to the eye appeared devoid of contrast. Fourier transforms of the images revealed the reciprocal lattice up to 3 angstrom resolution in favourable cases and up to 4 angstrom resolution for about half the crystals. The reciprocal-lattice spots showed structure, indicating that the ordering of the crystals was not uniform. Data processing revealed details at higher than 2 angstrom resolution and indicated the presence of multiple mosaic blocks within the crystal which could be separately processed. The prospects for full three-dimensional structure determination by electron imaging of protein three-dimensional nanocrystals are discussed.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Nano-diffraction of Biological Specimen (Abrahams)
UniBasel Contributors:Abrahams, Jan Pieter
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:06 Nov 2020 09:17
Deposited On:06 Nov 2020 09:17

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