Inelastic scattering and solvent scattering reduce dynamical diffraction in biological crystals

Latychevskaia, Tatiana and Abrahams, Jan Pieter. (2019) Inelastic scattering and solvent scattering reduce dynamical diffraction in biological crystals. Acta Crystallographica Section B-Structural Science Crystal Engineering and Materials, 75. pp. 523-531.

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Multi-slice simulations of electron diffraction by three-dimensional protein crystals have indicated that structure solution would be severely impeded by dynamical diffraction, especially when crystals are more than a few unit cells thick. In practice, however, dynamical diffraction turned out to be less of a problem than anticipated on the basis of these simulations. Here it is shown that two scattering phenomena, which are usually omitted from multi-slice simulations, reduce the dynamical effect: solvent scattering reduces the phase differences within the exit beam and inelastic scattering followed by elastic scattering results in diffusion of dynamical scattering out of Bragg peaks. Thus, these independent phenomena provide potential reasons for the apparent discrepancy between theory and practice in protein electron crystallography.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Nano-diffraction of Biological Specimen (Abrahams)
UniBasel Contributors:Abrahams, Jan Pieter
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:International Union of Crystallography
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:24 Mar 2020 13:26
Deposited On:24 Mar 2020 13:26

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