Purification of Biotinylated Proteins Using Single Walled Carbon Nanotube-Streptavidin Complexes

Wang, Run and Boleij, Marissa and Yin, Qu and Galjart, Niels and Lin, Bencai and Yuan, Ningyi and Zhou, Xiang and Tan, Ming and Ding, Jianning and Liu, Zunfeng and Abrahams, Jan Pieter. (2017) Purification of Biotinylated Proteins Using Single Walled Carbon Nanotube-Streptavidin Complexes. Journal of nanoscience and nanotechnology, 17 (2). pp. 926-931.

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In this study, Single walled carbon nanotube (SWNT)-streptavidin complexes were used to capture and purify biotinylated proteins, including bio-GFP and bio-DBS using a pull-down method. The purification conditions were systematically studied, including surface blocking of SWNT using chicken egg albumin (CEA), the ratio of SWNT-streptavidin complexes to the cell lysate, as well as the centrifugation speed. Optimization of the protein purification using SWNT-streptavidin complexes shows the possibility of carbon nanotubes as a promising candidate for protein purification applications. The SWNT-streptavidin could be used as a scaffold to analyze protein structure directly by cryo-transmission electron microscopy, which provides better understanding in protein-protein interactions and biological processes.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Nano-diffraction of Biological Specimen (Abrahams)
UniBasel Contributors:Abrahams, Jan Pieter
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:28 Mar 2020 16:24
Deposited On:28 Mar 2020 16:24

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