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Capillary electrophoresis with contactless conductivity detection for uric acid determination in biological fluids

Pormsila, W. and Krahenbuhl, S. and Hauser, P. C.. (2009) Capillary electrophoresis with contactless conductivity detection for uric acid determination in biological fluids. Analytica Chimica Acta, Vol. 636, pp. 224-228.

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Official URL: http://edoc.unibas.ch/dok/A5261863

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Abstract

The suitability of capillary electrophoresis (CE) with capacitively coupled contactless conductivity detection (C(4)D) for the direct determination of uric acid in human plasma and urine was investigated. It was found that a careful optimization of the buffer composition and pH was necessary to achieve selective determination in the complex sample matrices. An electrolyte solution consisting of 10mM 2-morpholinoethanesulfonic acid (MES), 10mM histidine and 0.1mM hexadecyltrimethylammonium bromide (CTAB), pH 6.0, was finally found suitable for use as running buffer for both sample matrices. The limit of detection (3 S/N) was determined as 3.3 microM. The linearity of the response was tested for the range between 10 and 500 microM and a correlation coefficient of 0.9996 was obtained. Intra- and inter-day variabilities were >10%. Quantitative analysis of urine and plasma samples showed a good correlation with the routine enzymatic method currently used at the University Hospital of Basel.
Faculties and Departments:03 Faculty of Medicine > Bereich Medizinische Fächer (Klinik) > Klinische Pharmakologie
03 Faculty of Medicine > Departement Klinische Forschung > Bereich Medizinische Fächer (Klinik) > Klinische Pharmakologie
03 Faculty of Medicine > Bereich Medizinische Fächer (Klinik) > Klinische Pharmakologie > Klinische Pharmakologie (Krähenbühl)
03 Faculty of Medicine > Departement Klinische Forschung > Bereich Medizinische Fächer (Klinik) > Klinische Pharmakologie > Klinische Pharmakologie (Krähenbühl)
05 Faculty of Science > Departement Chemie > Chemie > Analytische Chemie (Hauser)
05 Faculty of Science > Departement Pharmazeutische Wissenschaften
05 Faculty of Science > Departement Pharmazeutische Wissenschaften > Pharmazie > Pharmakologie (Krähenbühl)
UniBasel Contributors:Krähenbühl, Stephan and Hauser, Peter C. P. C.
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
ISSN:0003-2670
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:01 Mar 2013 11:11
Deposited On:22 Mar 2012 13:39

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