edoc

Profiling the Nucleobase and Structure Selectivity of Anticancer Drugs and other DNA Alkylating Agents by RNA Sequencing

Sauter, Basilius and Gillingham, Dennis. (2018) Profiling the Nucleobase and Structure Selectivity of Anticancer Drugs and other DNA Alkylating Agents by RNA Sequencing. ChemBioChem, 19 (15). pp. 1638-1642.

[img]
Preview
PDF - Accepted Version
1374Kb

Official URL: https://edoc.unibas.ch/72355/

Downloads: Statistics Overview

Abstract

Drugs that covalently modify DNA are components of most chemotherapy regimens, often serving as first‐line treatments. Classically, the reactivity and selectivity of DNA alkylating agents has been determined in vitro with short oligonucleotides. A statistically sound analysis of sequence preferences of alkylating agents is untenable with serial analysis methods because of the combinatorial explosion of sequence possibilities. Next‐generation sequencing (NGS) is ideally suited for the broad characterization of sequence or structure selectivities because it analyzes many sequences at once. Herein, NGS is used to report on the chemoselectivity of alkylating agents on RNA and this technology is applied to the previously uncharacterized alkylating agent trimethylsilyl diazomethane.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Organische Chemie (Gillingham)
UniBasel Contributors:Sauter, Basilius and Gillingham, Dennis
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Wiley
ISSN:1439-4227
e-ISSN:1439-7633
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Related URLs:
Identification Number:
Last Modified:04 Dec 2019 11:58
Deposited On:30 Oct 2019 15:42

Repository Staff Only: item control page