Expression and functional activity of the IL-8 receptor type CXCR1 and CXCR2 on human mast cells

Lippert, Undine and Artuc, Metin and Grützkau, Andreas and Möller, Annelie and Kenderessy-Szabo, Anna and Schadendorf, Dirk and Norgauer, Johannes and Hartmann, Karin and Schweitzer-Stenner, Reinhard and Zuberbier, Thorsten and Henz, Beate M. and Krüger-Krasagakes, Sabine. (1998) Expression and functional activity of the IL-8 receptor type CXCR1 and CXCR2 on human mast cells. Journal of Immunology, 161 (5). pp. 2600-2608.

Full text not available from this repository.

Official URL: https://edoc.unibas.ch/70864/

Downloads: Statistics Overview


To further elucidate mechanisms involved in mast cell accumulation at sites of cutaneous inflammation, we have studied the ability of human leukemic mast cells (HMC-1 cells) to express functionally active IL-8 receptors. Expression of mRNA for both types of IL-8 receptors (CXCR1 and CXCR2) was demonstrated by PCR and of both proteins by flow cytometry. Binding and competition studies with 125I-labeled IL-8 and its homologue melanoma growth stimulating activity (125I-labeled MGSA) revealed two specific binding sites for IL-8, K1 = 1.1 x 10(11) M(-1) and K2 = 5 x 10(7) M(-1); and for MGSA, K1 = 2.8 x 10(10) M(-1) and K2 = 5 x 10(7) M(-1). This finding was supported by a dose-dependent rise of cytosolic free calcium concentration ([Ca2+]i) induced by both chemokines and to a lesser extent by the homologue neutrophil-activating peptide-2 (NAP-2). A significant migratory response of human leukemic mast cells (HMC-1) was observed with all three chemokines at a range from 10(-8) M to 10(-9) M. Moreover, the formation of cellular F-actin was induced in a rapid, dose-dependent fashion, with a maximally 1.7-fold increase at 10(-7) M. Using postembedding immunoelectron microscopy, we could show the expression of CXCRI on the cytoplasmatic membrane of isolated human skin mast cells whereas CXCR2 was located in mast cell-specific granules. These findings demonstrate for the first time the functional expression of both types of IL-8 receptors on human mast cells, suggesting a role for their ligands during mast cell activation and recruitment.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Allergy and Immunity (Hartmann)
UniBasel Contributors:Hartmann, Karin
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Association of Immunologists
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:10 Nov 2020 14:16
Deposited On:10 Nov 2020 14:16

Repository Staff Only: item control page